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PDK4 Antibody (Center D98)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PDK4 Antibody (Center D98) AP7041F
    Anti-PDK4 Antibody (Center D98) at 1:1000 dilution + HL-60 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 46 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - PDK4 Antibody (Center D98) AP7041F
    Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PDK4 antibody (Center D98) (Cat.#AP7041f), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • IF - PDK4 Antibody (Center D98) AP7041F
    Confocal immunofluorescent analysis of PDK4 Antibody (Center D98)(Cat#AP7041f) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q16654
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 46469 Da
Antigen Region 83-111 aa
Additional Information
Gene ID 5166
Other Names [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial, Pyruvate dehydrogenase kinase isoform 4, PDK4, PDHK4
Target/Specificity This PDK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 83-111 amino acids from the Central region of human PDK4.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPDK4 Antibody (Center D98) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PDK4
Synonyms PDHK4
Function Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism in response to prolonged fasting and starvation. Plays an important role in maintaining normal blood glucose levels under starvation, and is involved in the insulin signaling cascade. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. In the fed state, mediates cellular responses to glucose levels and to a high-fat diet. Regulates both fatty acid oxidation and de novo fatty acid biosynthesis. Plays a role in the generation of reactive oxygen species. Protects detached epithelial cells against anoikis. Plays a role in cell proliferation via its role in regulating carbohydrate and fatty acid metabolism.
Cellular Location Mitochondrion matrix.
Tissue Location Ubiquitous; highest levels of expression in heart and skeletal muscle.
Research Areas
Citations (0)

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PDK4 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.


Rosa, G., et al., Obes. Res. 11(2):176-182 (2003).
Razeghi, P., et al., Cardiology 97(4):203-209 (2002).
Rowles, J., et al., J. Biol. Chem. 271(37):22376-22382 (1996).
Gudi, R., et al., J. Biol. Chem. 270(48):28989-28994 (1995).

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$ 295.00
$ 99.00
Cat# AP7041F
(40 western blots)
Availability: In Stock
Bulk Size
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