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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Protein Kinase A regulatory subunit I beta Antibody (N-term)   

Protein Kinase A regulatory subunit I beta Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - Protein Kinase A regulatory subunit I beta Antibody (N-term) AP7051a
    Western blot analysis of anti-PRKAR1B Pab (Cat. #AP7051a) in mouse liver tissue lysate. PRKAR1B (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
  • IHC-P - Protein Kinase A regulatory subunit I beta Antibody (N-term) AP7051a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P31321
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 43073 Da
Antigen Region 50-80 aa
Additional Information
Gene ID 5575
Other Names cAMP-dependent protein kinase type I-beta regulatory subunit, PRKAR1B
Target/Specificity This Protein Kinase A regulatory subunit I beta antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-80 amino acids from the N-terminal region of human Protein Kinase A regulatory subunit I beta.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsProtein Kinase A regulatory subunit I beta Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells.
Cellular Location Cell membrane.
Tissue Location Four types of regulatory chains are found: I- alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible
Research Areas
Citations (0)

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The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Cα, Cβ and Cγ, that each represent specific gene products. Cα and Cβ are closely related (93% amino acid sequence similari- ty), whereas Cγ displays 83% and 79% similarity to Cα and Cβ, respectively. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcrip- tion factor cAMP response element binding protein (CREB) on serine 133 which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the pol II transcription initiation complex.


Solberg, R., et al., Exp. Cell Res. 214(2):595-605 (1994).
Solberg, R., et al., Biochem. Biophys. Res. Commun. 176(1):166-172 (1991).

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Cat# AP7051a
(40 western blots)
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