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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   DNA PK (PRKDC) Antibody (C-term)   

DNA PK (PRKDC) Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - DNA PK (PRKDC) Antibody (C-term) AP7053b
    Anti-PRKDC Antibody (R4082) at 1:1000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 469 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - DNA PK (PRKDC) Antibody (C-term) AP7053b
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P78527
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 469089 Da
Antigen Region 4075-4104 aa
Additional Information
Gene ID 5591
Other Names DNA-dependent protein kinase catalytic subunit, DNA-PK catalytic subunit, DNA-PKcs, DNPK1, p460, PRKDC, HYRC, HYRC1
Target/Specificity This DNA PK (PRKDC) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4075~4104 amino acids from the C-terminal region of human PRKDC.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDNA PK (PRKDC) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms HYRC, HYRC1
Function Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 'Ser-588' and increasing CRY1 protein stability, most likely through an indirect machanism. Interacts with CRY1 and CRY2; negatively regulates CRY1 phosphorylation.
Cellular Location Nucleus. Nucleus, nucleolus.
Research Areas
Citations (0)

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The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties.[supplied by OMIM]


Goudelock, D.M., et al., J. Biol. Chem. 278(32):29940-29947 (2003).
Ding, Q., et al., Mol. Cell. Biol. 23(16):5836-5848 (2003).
Lucero, H., et al., J. Biol. Chem. 278(24):22136-22143 (2003).
Calsou, P., et al., J. Mol. Biol. 326(1):93-103 (2003).
Karpova, A.Y., et al., Proc. Natl. Acad. Sci. U.S.A. 99(5):2818-2823 (2002).

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$ 295.00
Cat# AP7053b
(40 western blots)
Availability: In Stock
Bulk Size
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