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Creatine Kinase B Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Creatine Kinase B Antibody (C-term) AP7059b
    Western blot analysis of CKB (arrow) using rabbit polyclonal CKB-C254 (Cat. #AP7059b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CKB gene.
    detail
  • WB - Creatine Kinase B Antibody (C-term) AP7059b
    The anti-CKB Pab (Cat. #AP7059b) is used in Western blot to detect CKB in Y79 cell lysate (Lane 1) and mouse colon tissue lysate (Lane 2).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P12277
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 42644 Da
Antigen Region 240-270 aa
Additional Information
Gene ID 1152
Other Names Creatine kinase B-type, B-CK, Creatine kinase B chain, CKB, CKBB
Target/Specificity This Creatine Kinase B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 240-270 amino acids from the C-terminal region of human Creatine Kinase B.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCreatine Kinase B Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CKB (HGNC:1991)
Synonyms CKBB
Function Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate) (PubMed:8186255). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa (Probable). Acts as a key regulator of adaptive thermogenesis as part of the futile creatine cycle: localizes to the mitochondria of thermogenic fat cells and acts by mediating phosphorylation of creatine to initiate a futile cycle of creatine phosphorylation and dephosphorylation (By similarity). During the futile creatine cycle, creatine and N-phosphocreatine are in a futile cycle, which dissipates the high energy charge of N- phosphocreatine as heat without performing any mechanical or chemical work (By similarity).
Cellular Location Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q04447}. Mitochondrion {ECO:0000250|UniProtKB:Q04447}. Cell membrane. Note=Localizes to the mitochondria of thermogenic fat cells via the internal MTS-like signal (iMTS-L) region {ECO:0000250|UniProtKB:Q04447}
Research Areas
Citations (0)
citation

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Background

Creatine Kinase (CK) is a dimeric enzyme composed of either M- or B-type subunits. The resulting isoenzymes are expressed at varying levels in different tissues. CK-BB, a cytoplasmic predominantly found in brain tissues, participates in energy homeostasis, reversibly catalyzing the transfer of a phosphate group between ATP and target proteins such as a creatine phosphate. CK-BB exists in normally neglible amounts in the serum of adults, overexpression of CK-BB is associated with cancers of the breast, ovary, prostate, colon, and in small-cell lung cancer. Global assessment of changes in serum levels of CK-BB, CK-MB and CK-MM, are used as a marker for myocardial infarction.

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$ 365.00
$ 140.00
Cat# AP7059b
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