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AK7 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - AK7 Antibody (C-term) AP7076d
    Western blot analysis of anti-AK7 Antibody (C-term) (Cat.#AP7076d) in K562 cell line lysates (35ug/lane).AK7(arrow) was detected using the purified Pab.
    detail
  • IHC-P - AK7 Antibody (C-term) AP7076d
    AK7 Antibody (C-term) (Cat. #AP7076d) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AK7 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q96M32
Other Accession Q95JP6, NP_689540
Reactivity Human
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 82658 Da
Antigen Region 629-656 aa
Additional Information
Gene ID 122481
Other Names Adenylate kinase 7, AK 7, ATP-AMP transphosphorylase 7, AK7
Target/Specificity This AK7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 629-656 amino acids from the C-terminal region of human AK7.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAK7 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AK7
Function Nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Has highest activity toward AMP, and weaker activity toward dAMP, CMP and dCMP. Also displays broad nucleoside diphosphate kinase activity. Involved in maintaining ciliary structure and function.
Cellular Location Cytoplasm, cytosol. Cell projection, cilium, flagellum Note=Detected along the full length of sperm flagellum, where it colocalizes with alpha-tubulin.
Tissue Location Expressed in sperm and airway epithelial cells (at protein level).
Research Areas
Citations (0)
citation

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Background

The adenylate kinases (AK) are a family of structurally and functionally related enzymes that catalyze a similar reaction, MgNTP + AMP = MgNDP + ADP (N = A or G). The AK enzymes are important for maintenance of homeostasis of the adenine and guanine nucleotide pools. AK1 is a cytosolic enzyme for which ATP is the substrate. AK2 catalyzes the same reaction as AK1, but it is localized in the mitochondrial intermembrane space. AK3 is present in the mitochondrial matrix and prefers GTP over ATP as the substrate.

References

Ota, T., et al., Nat. Genet. 36(1):40-45 (2004).

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$ 182.50
$ 70.00
Cat# AP7076d
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