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FN3K Antibody (N-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP7083a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
FN3K Antibody (N-term) - Product info | |
| Application | WB, IHC
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| Primary Accession | Q9H479 |
| Other Accession | NP_071441 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 35171 Da |
FN3K Antibody (N-term) - Additional info | |
| Gene ID 64122 | |
| Other Names FN3K; Fructosamine-3-kinase | |
| Target/Specificity This FN3K antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human FN3K. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions FN3K Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
FN3K Antibody (N-term) - Protein Information | |
| Name FN3K | |
| Function May initiate a process leading to the deglycation of fructoselysine and of glycated proteins. May play a role in the phosphorylation of 1-deoxy-1-morpholinofructose (DMF), fructoselysine, fructoseglycine, fructose and glycated lysozyme | |
| Tissue Location Expressed in erythrocytes. | |
FN3K Antibody (N-term) - Related products
AP7083a: FN3K Antibody (N-term)
AP7083b: FN3K Antibody (C-term)
DC07497: Human FN3K cDNA Clone
LY10419a: FN3K Over-expression Lysate
FN3K Antibody (N-term) - Application data
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Western blot analysis of anti-FN3K Antibody (N-term) (Cat.#AP7083a) in T47D cell line lysates (35ug/lane). FN3K (arrow) was detected using the purified Pab.
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Formalin-fixed and paraffin-embedded Human Diabetic kidney tissue reacted with the primary antibody at 1:50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. Data and protocol courtesy of Dr. Marc Halushka M.D. Ph.D. at Johns Hopkins Medical Institutions.
FN3K Antibody (N-term) - Research Areas
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BACKGROUND
FN3K catalyzes phosphorylation of fructosamines formed by glycation, the nonenzymatic reaction of glucose with primary amines followed by Amadori rearrangement. Phosphorylation of fructosamines may initiate metabolism of the modified amine and result in deglycation of glycated proteins. Human and mouse FN3K share 86% amino acid identity. GENE Purified FN3K catalyzes ATP-dependent phosphorylation of a synthetic fructosamine, 1-deoxy-1-morpholinofructose (DMF). Recombinant mouse and human FN3K, expressed in E. coli, catalyze phosphorylation of DMF, fructoselysine, fructoseglycine, and fructose in order of decreasing affinity. They also phosphorylate glycated lysozyme, but not unmodified lysozyme. In addition to ATP, FN3K can utilize GTP, CTP, and UTP as phosphate donors.
REFERENCES
Conner, J.R., et al., Biochem. Biophys. Res. Commun. 323(3):932-936 (2004). Delpierrre, G., et al., J. Biol. Chem. 279(26):27613-27620 (2004). Szwergold, B.S., et al., Diabetes 50(9):2139-2147 (2001). Delpierre, G., et al., Diabetes 49(10):1627-1634 (2000). Delpierre, G., et al., Biochem. Soc. Trans. 31 (PT 6), 1354-1357 (2003) (): ().
