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CERK Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CERK Antibody (C-term) AP7088b
    All lanes : Anti-CERK Antibody (C-term) at 1:1000 dilution Lane 1: SH-SY5Y whole cell lysate Lane 2: U-251 MG whole cell lysate Lane 3: HL-60 whole cell lysate Lane 4: Neuro-2a whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 60 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - CERK Antibody (C-term) AP7088b
    The anti-CERK Pab (Cat. #AP7088b) is used in Western blot to detect CERK in mouse heart tissue lysate (Lane 1) and A2058 cell lysate (Lane 2).
    detail
  • SPECIFICATION
  • CITATIONS: 5
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q8TCT0
Other Accession NP_073603
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 59977 Da
Antigen Region 487-516 aa
Additional Information
Gene ID 64781
Other Names Ceramide kinase, hCERK, Acylsphingosine kinase, Lipid kinase 4, LK4, CERK, KIAA1646
Target/Specificity This CERK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 487-516 amino acids from the C-terminal region of human CERK.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCERK Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CERK
Synonyms KIAA1646
Function Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate (PubMed:11956206, PubMed:16269826, PubMed:19168031). Acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8-dihydroceramide), to a lesser extent on C2- ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines (PubMed:11956206, PubMed:16269826, PubMed:19168031). Shows a greater preference for D-erythro isomer of ceramides (PubMed:16269826). Binds phosphoinositides (PubMed:19168031).
Cellular Location Cytoplasm. Cell membrane; Peripheral membrane protein
Tissue Location High level expression in heart, brain, skeletal muscle, kidney and liver; moderate in peripheral blood leukocytes and thymus; very low in spleen, small intestine, placenta and lung
Research Areas
Citations ( 0 )

Background

Ceramide kinases convert the sphingolipid metabolite ceramide into ceramide-1-phosphate, both key mediators of cellular apoptosis and survival. Ceramide metabolism plays an essential role in the viability of neuronal cells, the membranes of which are particularly rich in sphingolipids. CERK catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate. This enzyme acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8 dihydroceramide), and to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines. High level expression is noted in heart, brain, skeletal muscle, kidney and liver; moderate expression in peripheral blood leukocytes and thymus; and low expression in spleen, small intestine, placenta and lung.

References

J. Biol. Chem. 279 (17), 17570-17577 (2004)
J. Biol. Chem. 278 (40), 38206-38213 (2003)
J. Biol. Chem. 277 (26), 23294-23300 (2002)

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$ 182.50
$ 70.00
Cat# AP7088b
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