|Application ||WB, IHC-P, E|
|Calculated MW||19015 Da|
|Antigen Region||51-81 aa|
|Other Names||Nucleoside diphosphate kinase 3, NDK 3, NDP kinase 3, DR-nm23, Nucleoside diphosphate kinase C, NDPKC, nm23-H3, NME3|
|Target/Specificity||This NME3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-81 amino acids from the Central region of human NME3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||NME3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Probably has a role in normal hematopoiesis by inhibition of granulocyte differentiation and induction of apoptosis.|
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Provided below are standard protocols that you may find useful for product applications.
NME3 mRNA is preferentially expressed at early stages of myeloid differentiation of highly purified CD34(+) cells. Its constitutive expression in a myeloid precursor line, which is growth-factor dependent for both proliferation and differentiation, results in inhibition of granulocytic differentiation induced by granulocyte colony-stimulating factor and causes apoptotic cell death. These results appear consistent with a role for the NME3 gene in normal hematopoiesis and raise the possibility that its overexpression contributes to differentiation arrest, a feature of blastic transformation in chronic myelogenous leukemia.
Negroni, A., et al., Cell Death Differ. 7(9):843-850 (2000).
Martinez, R., et al., Cancer Res. 57(6):1180-1187 (1997).
Venturelli, D., et al., Proc. Natl. Acad. Sci. U.S.A. 92(16):7435-7439 (1995).
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