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PANK3 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PANK3 Antibody (Center) AP7161B
    Western blot analysis of anti-PANK3 (Cat. #AP7161b) in HepG2 cell line lysate (35ug/lane).PANK3(arrow) was detected using the purified Pab.
    detail
  • IHC-P - PANK3 Antibody (Center) AP7161B
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q9H999
Other Accession Q8R2W9, Q08DA5
Reactivity Human
Predicted Bovine, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 41094 Da
Antigen Region 150-181 aa
Additional Information
Gene ID 79646
Other Names Pantothenate kinase 3, hPanK3, Pantothenic acid kinase 3, PANK3
Target/Specificity This PANK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 150-181 amino acids from the Central region of human PANK3.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPANK3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PANK3
Function Catalyzes the phosphorylation of pantothenate to generate 4'- phosphopantothenate in the first and rate-determining step of coenzyme A (CoA) synthesis.
Cellular Location Cytoplasm {ECO:0000269|PubMed:23152917, ECO:0000305}
Tissue Location Highly expressed in the liver.
Research Areas
Citations (0)
citation

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Background

PANK3 belongs to the pantothenate kinase family. Pantothenate kinase is a key regulatory enzyme in the biosynthesis of coenzyme A (CoA) in bacteria and mammalian cells. It catalyzes the first committed step in the universal biosynthetic pathway leading to CoA and is itself subject to regulation through feedback inhibition by CoA. This family member is expressed most abundantly in the liver.

References

Zhou, B., et al., Nat. Genet. 28(4):345-349 (2001).
Robishaw, J.D., et al., Am. J. Physiol. 248 (1 PT 1), E1-E9 (1985) (): ().

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$ 182.50
$ 70.00
Cat# AP7161B
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