|Application ||WB, IHC-P, E|
|Calculated MW||35102 Da|
|Antigen Region||242-272 aa|
|Other Names||Pyridoxal kinase, Pyridoxine kinase, PDXK, C21orf124, C21orf97, PKH, PNK|
|Target/Specificity||This PDXK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 242-272 amino acids from the C-terminal region of human PDXK.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PDXK Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||C21orf124, C21orf97, PKH, PNK|
|Function||Required for synthesis of pyridoxal-5-phosphate from vitamin B6.|
|Tissue Location||Ubiquitous. Isoform 3 is detected in adult testis and spermatozoa|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Pyridoxal kinase (PDXK) converts vitamin B6 to pyridoxal-5-phosphate (PLP), an essential cofactor in the intermediate metabolism of amino acids and neurotransmitters. The PDXK gene encodes a 312-amino acid polypeptide, and expression of the cDNA reveals pyridoxal kinase activity. Northern blot analysis revealed that a major 1.5-kb PDXK transcript is expressed in all tissues tested. The expression of PDXK shows circadian oscillations. The expression of Pdxk in mouse liver and brain is regulated by the 3 PAR bZIP transcription factors, Dbp, Hlf, and Tef, which also show circadian oscillations in expression. Mice devoid of all 3 transcription factors show decreased levels of brain PLP, serotonin, and dopamine, and are highly susceptible to frequently lethal generalized spontaneous and audiogenic epilepsies.
Shin, J.H., et al., Neurochem. Int. 45(1):73-79 (2004).
Lee, H.S., et al., Mol. Cells 10(4):452-459 (2000).
Laine-Cessac, P., et al., Biochem. Pharmacol. 54(8):863-870 (1997).
Hanna, M.C., et al., J. Biol. Chem. 272(16):10756-10760 (1997).
Zhang, Z., et al., J. Nutr. 10(1):53-59 (1993).
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.