|Application ||WB, E|
|Calculated MW||44796 Da|
|Antigen Region||305-333 aa|
|Other Names||Phosphoglycerate kinase 2, Phosphoglycerate kinase, testis specific, PGK2, PGKB|
|Target/Specificity||This PGK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 305-333 amino acids from the C-terminal region of human PGK2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PGK2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Essential for sperm motility and male fertility (PubMed:26677959). Not required for the completion of spermatogenesis (By similarity).|
|Tissue Location||Mainly found in round spermatids. Localized on the principle piece in the sperm (at protein level). Testis- specific. Expression significantly decreased in the testis of elderly men.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
PGK2 is a form of phosphoglycerate kinase unique to spermatozoa. The autosomal PGK2 gene lacks introns and contains characteristics of a processed gene. The conservation of function in this processed PGK2 gene and its tissue-specific expression in spermatogenesis suggests that it exists as a compensatory response to the inactivation of the X-linked PGK1 gene in spermatogenic cells before meiosis.
Zhang, L.P., et al., Biol. Reprod. 60(6):1329-1337 (1999).
McCarrey, J.R., et al., Dev. Genet. 19(4):321-332 (1996).
Blanche, H., et al., Genomics 9(3):420-428 (1991).
McCarrey, J.R., et al., Nature 326(6112):501-505 (1987).
Michelson, A.M., et al., J. Biol. Chem. 260(11):6982-6992 (1985).
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.