PGK2 Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
---|---|
Primary Accession | P07205 |
Other Accession | Q4R3K4 |
Reactivity | Human |
Predicted | Monkey |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 44796 Da |
Antigen Region | 305-333 aa |
Gene ID | 5232 |
---|---|
Other Names | Phosphoglycerate kinase 2, Phosphoglycerate kinase, testis specific, PGK2, PGKB |
Target/Specificity | This PGK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 305-333 amino acids from the C-terminal region of human PGK2. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | PGK2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | PGK2 |
---|---|
Synonyms | PGKB |
Function | Essential for sperm motility and male fertility (PubMed:26677959). Not required for the completion of spermatogenesis (By similarity). |
Cellular Location | Cytoplasm. |
Tissue Location | Mainly found in round spermatids. Localized on the principle piece in the sperm (at protein level). Testis-specific Expression significantly decreased in the testis of elderly men |
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Provided below are standard protocols that you may find useful for product applications.
Background
PGK2 is a form of phosphoglycerate kinase unique to spermatozoa. The autosomal PGK2 gene lacks introns and contains characteristics of a processed gene. The conservation of function in this processed PGK2 gene and its tissue-specific expression in spermatogenesis suggests that it exists as a compensatory response to the inactivation of the X-linked PGK1 gene in spermatogenic cells before meiosis.
References
Zhang, L.P., et al., Biol. Reprod. 60(6):1329-1337 (1999).
McCarrey, J.R., et al., Dev. Genet. 19(4):321-332 (1996).
Blanche, H., et al., Genomics 9(3):420-428 (1991).
McCarrey, J.R., et al., Nature 326(6112):501-505 (1987).
Michelson, A.M., et al., J. Biol. Chem. 260(11):6982-6992 (1985).
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