|Application ||WB, E|
|Calculated MW||44796 Da|
|Antigen Region||305-333 aa|
|Other Names||Phosphoglycerate kinase 2, Phosphoglycerate kinase, testis specific, PGK2, PGKB|
|Target/Specificity||This PGK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 305-333 amino acids from the C-terminal region of human PGK2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PGK2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Tissue Location||Testis specific.|
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Provided below are standard protocols that you may find useful for product applications.
PGK2 is a form of phosphoglycerate kinase unique to spermatozoa. The autosomal PGK2 gene lacks introns and contains characteristics of a processed gene. The conservation of function in this processed PGK2 gene and its tissue-specific expression in spermatogenesis suggests that it exists as a compensatory response to the inactivation of the X-linked PGK1 gene in spermatogenic cells before meiosis.
Zhang, L.P., et al., Biol. Reprod. 60(6):1329-1337 (1999).
McCarrey, J.R., et al., Dev. Genet. 19(4):321-332 (1996).
Blanche, H., et al., Genomics 9(3):420-428 (1991).
McCarrey, J.R., et al., Nature 326(6112):501-505 (1987).
Michelson, A.M., et al., J. Biol. Chem. 260(11):6982-6992 (1985).
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