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PIP5K3 Antibody (Center)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
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| Catalog # | Size | Availability | Price | |
| AP7172a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
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- Backgrounds
PIP5K3 Antibody (Center) - Product info | |
| Application | WB, IHC
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| Primary Accession | Q9Y2I7 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 237136 Da |
PIP5K3 Antibody (Center) - Additional info | |
| Gene ID 200576 | |
| Other Names PIKFYVE; KIAA0981; PIP5K3; 1-phosphatidylinositol 3-phosphate 5-kinase; FYVE finger-containing phosphoinositide kinase; PIKfyve; Phosphatidylinositol 3-phosphate 5-kinase type III | |
| Target/Specificity This PIP5K3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19~48 amino acids from the center region of human PIP5K3. | |
| Dilution WB~~1:50~100WB~~1:1000 IHC~~1:50~100 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions PIP5K3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. | |
PIP5K3 Antibody (Center) - Protein Information | |
| Name PIKFYVE | |
| Synonyms KIAA0981, PIP5K3 | |
| Function The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Catalyzes the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo- inositol ring, to form phosphatidylinositol 3,5-bisphosphate Required for endocytic-vacuolar pathway and nuclear migration Plays a role in the biogenesis of endosome carrier vesicles (ECV)/ multivesicular bodies (MVB) transport intermediates from early endosomes | |
| Cellular Location Endosome membrane. Note=Mainly associated with membranes of the late endocytic pathway | |
PIP5K3 Antibody (Center) - Related products
AP7172a: PIP5K3 Antibody (Center)
RI14003: PIKFYVE predesign siRNA
DC06378: Human PIP5K3 cDNA Clone
LY11534a: PIP5K3 Over-expression Lysate
BP7172a: PIP5K3 Antibody (Center) Blocking Peptide
BP7172c: PIP5K3 Antibody (C-term) Blocking Peptide
PIP5K3 Antibody (Center) - Application data
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Western blot analysis of PIP5K3 (arrow) using rabbit polyclonal PIP5K3 Antibody (Center) (Cat.#AP7172a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIP5K3 gene (Lane 2) (Origene Technologies).
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
PIP5K3 Antibody (Center) - Research Areas
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BACKGROUND
PIP5K3 belongs to a large family of lipid kinases that alter the phosphorylation status of intracellular phosphatidylinositol. Signaling by phosphorylated species of phosphatidylinositol regulates diverse cellular processes, including membrane trafficking and cytoskeletal reorganization.
REFERENCES
Sbrissa, D., et al., Mol. Cell. Biol. 24(23):10437-10447 (2004). Yang, S.A., et al., J. Biol. Chem. 279(40):42331-42336 (2004). Ikonomov, O.C., et al., J. Biol. Chem. 278(51):50863-50871 (2003). Ikonomov, O.C., et al., Mol. Biol. Cell 14(11):4581-4591 (2003). Sbrissa, D., et al., J. Biol. Chem. 277(8):6073-6079 (2002).
