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RBKS (RBSK) Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - RBKS (RBSK) Antibody (N-term) AP7182a
    Western blot analysis of anti-RBSK Pab(Cat. #AP7182a) in HepG2 cell line lysate (35ug/lane). RBSK(arrow) was detected using the purified Pab.
    detail
  • IHC-P - RBKS (RBSK) Antibody (N-term) AP7182a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q9H477
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 34143 Da
Antigen Region 16-46 aa
Additional Information
Gene ID 64080
Other Names Ribokinase, RBKS, RBSK
Target/Specificity This RBKS (RBSK) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-46 amino acids from the N-terminal region of human RBKS (RBSK).
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRBKS (RBSK) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RBKS {ECO:0000255|HAMAP-Rule:MF_03215}
Synonyms RBSK
Function Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5-phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway.
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:P25332, ECO:0000255|HAMAP-Rule:MF_03215}. Nucleus {ECO:0000250|UniProtKB:P25332, ECO:0000255|HAMAP-Rule:MF_03215}
Research Areas
Citations (0)
citation

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Background

RBSK belongs to the pfkB family of carbohydrate kinases. It phosphorylates ribose to form ribose-5-phosphate in the presence of ATP and magnesium as a first step in ribose metabolism.

References

Bork, P., et al., Protein Sci. 2(1):31-40 (1993).

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$ 182.50
$ 70.00
Cat# AP7182a
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