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RFK Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| FC, WB, IHC-P, E |
|---|---|
| Primary Accession | Q969G6 |
| Other Accession | Q8CFV9 |
| Reactivity | Human |
| Predicted | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 17623 Da |
| Antigen Region | 1-30 aa |
| Gene ID | 55312 |
|---|---|
| Other Names | Riboflavin kinase, ATP:riboflavin 5'-phosphotransferase, Flavokinase, RFK |
| Target/Specificity | This RFK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human RFK. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | RFK Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | RFK |
|---|---|
| Function | Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN), hence rate-limiting enzyme in the synthesis of FAD. Essential for TNF-induced reactive oxygen species (ROS) production. Through its interaction with both TNFRSF1A and CYBA, physically and functionally couples TNFRSF1A to NADPH oxidase. TNF- activation of RFK may enhance the incorporation of FAD in NADPH oxidase, a critical step for the assembly and activation of NADPH oxidase. |
| Cellular Location | Cytoplasm. |
| Tissue Location | Detected in brain, placenta and urinary bladder. |
Research Areas
Citations ( 0 )
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
RFK (riboflavin kinase) catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN).
References
Karthikeyan, S., et al., Biochemistry 42(43):12532-12538 (2003).
Karthikeyan, S., et al., Structure (Camb.) 11(3):265-273 (2003).
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$ 182.50
$ 70.00
Cat# AP7183a
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