|Application ||IHC-P, WB, FC, E|
|Calculated MW||17623 Da|
|Antigen Region||1-30 aa|
|Other Names||Riboflavin kinase, ATP:riboflavin 5'-phosphotransferase, Flavokinase, RFK|
|Target/Specificity||This RFK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human RFK.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RFK Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN), hence rate-limiting enzyme in the synthesis of FAD. Essential for TNF-induced reactive oxygen species (ROS) production. Through its interaction with both TNFRSF1A and CYBA, physically and functionally couples TNFRSF1A to NADPH oxidase. TNF-activation of RFK may enhance the incorporation of FAD in NADPH oxidase, a critical step for the assembly and activation of NADPH oxidase.|
|Tissue Location||Detected in brain, placenta and urinary bladder|
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Provided below are standard protocols that you may find useful for product applications.
RFK (riboflavin kinase) catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN).
Karthikeyan, S., et al., Biochemistry 42(43):12532-12538 (2003).
Karthikeyan, S., et al., Structure (Camb.) 11(3):265-273 (2003).
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