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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   RFK Antibody (N-term)   

RFK Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • IHC-P - RFK Antibody (N-term) AP7183a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • WB - RFK Antibody (N-term) AP7183a
    Western blot analysis of RFK (arrow) using rabbit polyclonal RFK Antibody (C15) (Cat. #AP7183a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RFK gene.
  • FC - RFK Antibody (N-term) AP7183a
    RFK Antibody (N-term) (Cat. #AP7183a) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q969G6
Other Accession Q8CFV9
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 17623 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 55312
Other Names Riboflavin kinase, ATP:riboflavin 5'-phosphotransferase, Flavokinase, RFK
Target/Specificity This RFK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human RFK.
Dilution IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRFK Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RFK
Function Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN), hence rate-limiting enzyme in the synthesis of FAD. Essential for TNF-induced reactive oxygen species (ROS) production. Through its interaction with both TNFRSF1A and CYBA, physically and functionally couples TNFRSF1A to NADPH oxidase. TNF-activation of RFK may enhance the incorporation of FAD in NADPH oxidase, a critical step for the assembly and activation of NADPH oxidase.
Cellular Location Cytoplasm.
Tissue Location Detected in brain, placenta and urinary bladder
Research Areas
Citations (0)

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RFK (riboflavin kinase) catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN).


Karthikeyan, S., et al., Biochemistry 42(43):12532-12538 (2003).
Karthikeyan, S., et al., Structure (Camb.) 11(3):265-273 (2003).

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Cat# AP7183a
(40 western blots)
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