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RIPK3 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - RIPK3 Antibody (Center) AP7184b
    Western blot analysis of lysates from HT-29, K562 cell line (from left to right), using RIPK3 Antibody Center(Cat. #AP7184b). AP7184b was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
  • WB - RIPK3 Antibody (Center) AP7184b
    Western blot analysis of RIP3 (RIPK3) Antibody (Center) (Cat.# AP7184b) in Hela cell line lysates (35ug/lane). RIPK3 (arrow) was detected using the purified Pab.
  • WB - RIPK3 Antibody (Center) AP7184b
    Western blot analysis of RIP3 (RIPK3) Antibody (Center) (Cat.# AP7184b) in mouse cerebellum tissue lysates (35ug/lane). RIPK3 (arrow) was detected using the purified Pab.
  • IHC-P - RIPK3 Antibody (Center) AP7184b
    Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with RIP3 (RIPK3) antibody (Center) (Cat.# AP7184b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9Y572
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 56887 Da
Antigen Region 61-91 aa
Additional Information
Gene ID 11035
Other Names Receptor-interacting serine/threonine-protein kinase 3, RIP-like protein kinase 3, Receptor-interacting protein 3, RIP-3, RIPK3, RIP3
Target/Specificity This RIPK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 61-91 amino acids from the Central region of human RIPK3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRIPK3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RIPK3
Synonyms RIP3
Function Essential for necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members. Upon induction of necrosis, RIPK3 interacts with, and phosphorylates RIPK1 and MLKL to form a necrosis-inducing complex. RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL. These metabolic enzymes may eventually stimulate the tricarboxylic acid cycle and oxidative phosphorylation, which could result in enhanced ROS production.
Cellular Location Cytoplasm, cytosol. Cell membrane Mitochondrion
Tissue Location Highly expressed in the pancreas. Detected at lower levels in heart, placenta, lung and kidney. Isoform 3 is significantly increased in colon and lung cancers
Research Areas
Citations (0)

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The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor.


Yu P.W., Huang B.C.B., Shen M., Quast J., Chan E., Xu X., Nolan G.P., Payan D.G., Luo Y. Curr. Biol. 9:539-542(1999).
Sun X., Lee J., Navas T., Baldwin D.T., Stewart T.A., Dixit V.M.; J. Biol. Chem. 274:16871-16875(1999).

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Cat# AP7184b
(40 western blots)
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