|Application ||WB, E|
|Other Accession||P52925, P17741, P40673|
|Predicted||Bovine, Pig, Rat|
|Calculated MW||24034 Da|
|Antigen Region||92-118 aa|
|Other Names||High mobility group protein B2, High mobility group protein 2, HMG-2, HMGB2, HMG2|
|Target/Specificity||This HMGB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92-118 amino acids from the Central region of human HMGB2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HMGB2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS) (By similarity).|
|Cellular Location||Nucleus. Chromosome.|
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Provided below are standard protocols that you may find useful for product applications.
HMGB2 is a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination.
Majumdar A., Brown D. 19:6643-6643(1991)
Shirakawa H., Yoshida M.J.. 267:6641-6645(1992)
Alexandre S., Li W.W.Nucleic Acids Res. 20:6413-6413(1992)
Fan Z., Beresford P.J.Mol. Cell. Biol. 22:2810-2820(2002)
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