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RIOK1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - RIOK1 Antibody (C-term) AP7200b
    Western blot analysis of anti-RIOK1 Pab in A375 cell line lysate. RIOK1(arrow) was detected using the purified Pab.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9BRS2
Other Accession Q922Q2
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 65583 Da
Antigen Region 522-551 aa
Additional Information
Gene ID 83732
Other Names Serine/threonine-protein kinase RIO1, RIO kinase 1, RIOK1
Target/Specificity This RIOK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 522-551 amino acids from the C-terminal region of human RIOK1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRIOK1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RIOK1 (HGNC:18656)
Synonyms RIO1
Function Involved in the final steps of cytoplasmic maturation of the 40S ribosomal subunit. Involved in processing of 18S-E pre-rRNA to the mature 18S rRNA. Required for the recycling of NOB1 and PNO1 from the late 40S precursor (PubMed:22072790). The association with the very late 40S subunit intermediate may involve a translation-like checkpoint point cycle preceeding the binding to the 60S ribosomal subunit (By similarity). Despite the protein kinase domain is proposed to act predominantly as an ATPase (By similarity). The catalytic activity regulates its dynamic association with the 40S subunit (By similarity). In addition to its role in ribosomal biogenesis acts as an adapter protein by recruiting NCL/nucleolin the to PRMT5 complex for its symmetrical methylation (PubMed:21081503).
Cellular Location Cytoplasm, cytosol.
Research Areas
Citations (0)
citation

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References

Line, A., et al., Cancer Immunol. Immunother. 51(10):574-582 (2002).

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$ 182.50
$ 70.00
Cat# AP7200b
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