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CAMK1D Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CAMK1D Antibody (N-term) AP7204b
    Western blot analysis of CAMK1D (arrow) using rabbit polyclonal CAMK1D Antibody (N-term) (Cat. #AP7204b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CAMK1D gene (Lane 2).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q8IU85
Other Accession Q8BW96
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 42914 Da
Antigen Region 39-69 aa
Additional Information
Gene ID 57118
Other Names Calcium/calmodulin-dependent protein kinase type 1D, CaM kinase I delta, CaM kinase ID, CaM-KI delta, CaMKI delta, CaMKID, CaMKI-like protein kinase, CKLiK, CAMK1D, CAMKID
Target/Specificity This CAMK1D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-69 amino acids from the N-terminal region of human CAMK1D.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCAMK1D Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CAMK1D
Synonyms CAMKID
Function Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, activates CREB-dependent gene transcription, regulates calcium- mediated granulocyte function and respiratory burst and promotes basal dendritic growth of hippocampal neurons. In neutrophil cells, required for cytokine-induced proliferative responses and activation of the respiratory burst. Activates the transcription factor CREB1 in hippocampal neuron nuclei. May play a role in apoptosis of erythroleukemia cells. In vitro, phosphorylates transcription factor CREM isoform Beta.
Cellular Location Cytoplasm. Nucleus. Note=Predominantly cytoplasmic. Nuclear localization increases upon activation by KCl treatment in hippocampal neurons
Tissue Location Widely expressed. Highly and mostly expressed in polymorphonuclear leukocytes (neutrophilic and eosinophilic granulocytes) while little or no expression is observed in monocytes and lymphocytes.
Research Areas
Citations (0)
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Background

CAMK1-like protein kinase belongs to a proposed calcium-triggered signaling cascade. This protein is expressed in polymorphonuclear leukocytes (PMNs) and may be part of the chemokine signal transduction pathway that regulates granulocyte function. CAMK1-like protein kinase may play a role in apoptosis of erythroleukemia cells. It activates MAP kinase MAPK3, and in vitro, phosphorylates transcription factor CREM isoform Beta and probably CREB1.

References

Verploegen, S., et al., Blood 96(9):3215-3223 (2000).
Ishikawa, Y., et al., FEBS Lett. 550 (1-3), 57-63 (2003).

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$ 365.00
$ 140.00
Cat# AP7204b
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