- CITATIONS: 2
|Application ||WB, IHC-P, E|
|Calculated MW||93219 Da|
|Antigen Region||828-859 aa|
|Other Names||Mitogen-activated protein kinase kinase kinase 12, Dual leucine zipper bearing kinase, DLK, Leucine-zipper protein kinase, ZPK, MAPK-upstream kinase, MUK, Mixed lineage kinase, MAP3K12, ZPK|
|Target/Specificity||This ZPK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 828-859 amino acids from the C-terminal region of human ZPK.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ZPK Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May be an activator of the JNK/SAPK pathway. Phosphorylates beta-casein, histone 1 and myelin basic protein in vitro.|
|Cellular Location||Cytoplasm. Membrane; Peripheral membrane protein|
|Tissue Location||Highly expressed in brain and kidney.|
Provided below are standard protocols that you may find useful for product applications.
ZPK is a member of serine/threonine protein kinase family. This kinase contains a leucine-zipper domain, and is predominately expressed in neuronal cells. The phosphorylation state of this kinase in synaptic terminals was shown to be regulated by membrane depolarization via calcineurin. This kinase forms heterodimers with leucine zipper containing transcription factors, such as cAMP responsive element binding protein (CREB) and MYC, and thus may play a regulatory role in PKA or retinoic acid induced neuronal differentiation.
Itoh, A., et al., Biochem. Biophys. Res. Commun. 313(3):612-618 (2004).
Fukuyama, K., et al., J. Biol. Chem. 275(28):21247-21254 (2000).
Reddy, U.R., et al., Oncogene 18(31):4474-4484 (1999).
Mata, M., et al., J. Biol. Chem. 271(28):16888-16896 (1996).
Hirai, S., et al., Oncogene 12(3):641-650 (1996).
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