|Application ||WB, IHC-P, E|
|Calculated MW||89631 Da|
|Antigen Region||156-185 aa|
|Other Names||N-terminal kinase-like protein, Coated vesicle-associated kinase of 90 kDa, SCY1-like protein 1, Telomerase regulation-associated protein, Telomerase transcriptional element-interacting factor, Teratoma-associated tyrosine kinase, SCYL1, CVAK90, GKLP, NTKL, TAPK, TEIF, TRAP|
|Target/Specificity||This SCYL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-185 amino acids from the N-terminal region of human SCYL1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SCYL1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||CVAK90, GKLP, NTKL, TAPK, TEIF, TRAP|
|Function||Regulates COPI-mediated retrograde traffic. Has no detectable kinase activity in vitro.|
|Cellular Location||Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Endoplasmic reticulum-Golgi intermediate compartment. Golgi apparatus, cis-Golgi network. Note=Localized to the Endoplasmic reticulum-Golgi intermediate and cis-Golgi in an ARF1-independent manner Isoform 2: Cytoplasm. Note=Cytoplasmic throughout the cell cycle Isoform 6: Nucleus|
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Provided below are standard protocols that you may find useful for product applications.
SCYL1 forms multimers following transfection into COS-7 cells. SCYL1 forms a 300-kD trimer using crosslinking reagents. Biochemical analysis revealed no phosphorylation or autophosphorylation activity.The 707-amino acid SCYL1 variant, variant 2, localized to centrosomes during mitosis. During interphase, fluorescence-tagged variant 2 localized in the cytoplasm as well as centrosomes. However, at the beginning of mitosis, the fluorescence appeared as a pair of bright nuclear foci that followed centrosome localization throughout mitosis, while maintaining diffuse cytoplasmic labeling. Endogenous variant 2 in HeLa cells showed a similar staining pattern. Centrosomal localization was independent of microtubules.
Tang, Z., et al., Biochem. Biophys. Res. Commun. 324(4):1324-1332 (2004).
Kato, M., et al., Genomics 79(6):760-767 (2002).
Liu, S.C., et al., Biochim. Biophys. Acta 1517(1):148-152 (2000).
van Asseldonk, M., et al., Genomics 66(1):35-42 (2000).
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