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SCYL1 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
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- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | Q96KG9 |
| Other Accession | A6QLH6 |
| Reactivity | Human |
| Predicted | Bovine |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 89631 Da |
| Antigen Region | 156-185 aa |
| Gene ID | 57410 |
|---|---|
| Other Names | N-terminal kinase-like protein, Coated vesicle-associated kinase of 90 kDa, SCY1-like protein 1, Telomerase regulation-associated protein, Telomerase transcriptional element-interacting factor, Teratoma-associated tyrosine kinase, SCYL1, CVAK90, GKLP, NTKL, TAPK, TEIF, TRAP |
| Target/Specificity | This SCYL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-185 amino acids from the N-terminal region of human SCYL1. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | SCYL1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SCYL1 |
|---|---|
| Synonyms | CVAK90, GKLP, NTKL, TAPK, TEIF, TRAP |
| Function | Regulates COPI-mediated retrograde protein traffic at the interface between the Golgi apparatus and the endoplasmic reticulum (PubMed:18556652). Involved in the maintenance of the Golgi apparatus morphology (PubMed:26581903). Has no detectable kinase activity in vitro (PubMed:18556652). |
| Cellular Location | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Endoplasmic reticulum-Golgi intermediate compartment Golgi apparatus, cis-Golgi network Note=Localized to the Endoplasmic reticulum-Golgi intermediate and cis- Golgi in an ARF1-independent manner [Isoform 2]: Cytoplasm. Note=Cytoplasmic throughout the cell cycle [Isoform 6]: Nucleus |
| Tissue Location | Ubiquitous.. |
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Provided below are standard protocols that you may find useful for product applications.
Background
SCYL1 forms multimers following transfection into COS-7 cells. SCYL1 forms a 300-kD trimer using crosslinking reagents. Biochemical analysis revealed no phosphorylation or autophosphorylation activity.The 707-amino acid SCYL1 variant, variant 2, localized to centrosomes during mitosis. During interphase, fluorescence-tagged variant 2 localized in the cytoplasm as well as centrosomes. However, at the beginning of mitosis, the fluorescence appeared as a pair of bright nuclear foci that followed centrosome localization throughout mitosis, while maintaining diffuse cytoplasmic labeling. Endogenous variant 2 in HeLa cells showed a similar staining pattern. Centrosomal localization was independent of microtubules.
References
Tang, Z., et al., Biochem. Biophys. Res. Commun. 324(4):1324-1332 (2004).
Kato, M., et al., Genomics 79(6):760-767 (2002).
Liu, S.C., et al., Biochim. Biophys. Acta 1517(1):148-152 (2000).
van Asseldonk, M., et al., Genomics 66(1):35-42 (2000).
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