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SCYL1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - SCYL1 Antibody (N-term) AP7215a
    SCYL1 Antibody (N-term) (Cat. #AP7215a) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the SCYL1 antibody detected the SCYL1 protein (arrow).
  • IHC-P - SCYL1 Antibody (N-term) AP7215a
    SCYL1 Antibody (N-term) (Cat. #AP7215a)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SCYL1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q96KG9
Other Accession A6QLH6
Reactivity Human
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 89631 Da
Antigen Region 156-185 aa
Additional Information
Gene ID 57410
Other Names N-terminal kinase-like protein, Coated vesicle-associated kinase of 90 kDa, SCY1-like protein 1, Telomerase regulation-associated protein, Telomerase transcriptional element-interacting factor, Teratoma-associated tyrosine kinase, SCYL1, CVAK90, GKLP, NTKL, TAPK, TEIF, TRAP
Target/Specificity This SCYL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-185 amino acids from the N-terminal region of human SCYL1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSCYL1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SCYL1
Function Regulates COPI-mediated retrograde traffic. Has no detectable kinase activity in vitro.
Cellular Location Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Endoplasmic reticulum-Golgi intermediate compartment. Golgi apparatus, cis-Golgi network. Note=Localized to the Endoplasmic reticulum-Golgi intermediate and cis-Golgi in an ARF1-independent manner Isoform 2: Cytoplasm. Note=Cytoplasmic throughout the cell cycle Isoform 6: Nucleus
Tissue Location Ubiquitous..
Research Areas
Citations (0)

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SCYL1 forms multimers following transfection into COS-7 cells. SCYL1 forms a 300-kD trimer using crosslinking reagents. Biochemical analysis revealed no phosphorylation or autophosphorylation activity.The 707-amino acid SCYL1 variant, variant 2, localized to centrosomes during mitosis. During interphase, fluorescence-tagged variant 2 localized in the cytoplasm as well as centrosomes. However, at the beginning of mitosis, the fluorescence appeared as a pair of bright nuclear foci that followed centrosome localization throughout mitosis, while maintaining diffuse cytoplasmic labeling. Endogenous variant 2 in HeLa cells showed a similar staining pattern. Centrosomal localization was independent of microtubules.


Tang, Z., et al., Biochem. Biophys. Res. Commun. 324(4):1324-1332 (2004).
Kato, M., et al., Genomics 79(6):760-767 (2002).
Liu, S.C., et al., Biochim. Biophys. Acta 1517(1):148-152 (2000).
van Asseldonk, M., et al., Genomics 66(1):35-42 (2000).

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$ 295.00
$ 99.00
Cat# AP7215a
(40 western blots)
Availability: In Stock
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