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DAPK1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - DAPK1 Antibody (C-term) AP7217b
    The DAPK1 polyclonal antibody (Cat. #AP7217b) is used in Western blot to detect DAPK1 in mouse liver tissue lysate.
  • IHC-P - DAPK1 Antibody (C-term) AP7217b
    Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with DAPK1 antibody (C-term) (Cat.#AP7217b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • IF - DAPK1 Antibody (C-term) AP7217b
    Confocal immunofluorescent analysis of DAP Kinase 1 (DAPK1) Antibody (C-term) (Cat. #AP7217b) with A549 cell followed by Alexa Fluor® 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P53355
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 160046 Da
Antigen Region 1360-1389 aa
Additional Information
Gene ID 1612
Other Names Death-associated protein kinase 1, DAP kinase 1, DAPK1, DAPK
Target/Specificity This DAPK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1360-1389 amino acids from the C-terminal region of human DAPK1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDAPK1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DAPK1
Synonyms DAPK
Function Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. Phosphorylates TPM1, enhancing stress fiber formation in endothelial cells. Phosphorylates STX1A and significantly decreases its binding to STXBP1. Phosphorylates PRKD1 and regulates JNK signaling by binding and activating PRKD1 under oxidative stress. Phosphorylates BECN1, reducing its interaction with BCL2 and BCL2L1 and promoting the induction of autophagy. Phosphorylates TSC2, disrupting the TSC1-TSC2 complex and stimulating mTORC1 activity in a growth factor-dependent pathway. Phosphorylates RPS6, MYL9 and DAPK3. Acts as a signaling amplifier of NMDA receptors at extrasynaptic sites for mediating brain damage in stroke. Cerebral ischemia recruits DAPK1 into the NMDA receptor complex and it phosphorylates GRINB at Ser-1303 inducing injurious Ca(2+) influx through NMDA receptor channels, resulting in an irreversible neuronal death. Required together with DAPK3 for phosphorylation of RPL13A upon interferon-gamma activation which is causing RPL13A involvement in transcript- selective translation inhibition.
Cellular Location Isoform 1: Cytoplasm. Cytoplasm, cytoskeleton. Note=Colocalizes with MAP1B in the microtubules and cortical actin fibers
Tissue Location Isoform 2 is expressed in normal intestinal tissue as well as in colorectal carcinomas
Research Areas
Citations (0)

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Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death (apoptosis). DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate.


Shohat, G., et al., J. Biol. Chem. 276(50):47460-47467 (2001).
Inbal, B., et al., Mol. Cell. Biol. 20(3):1044-1054 (2000).
Deiss, L.P., et al., Genes Dev. 9(1):15-30 (1995).

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Cat# AP7217b
(40 western blots)
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