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MAPK12 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - MAPK12 Antibody (Center) AP7224C
    All lanes : Anti-MAPK12 Antibody (P354) at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: Jurkat whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - MAPK12 Antibody (Center) AP7224C
    Western blot analysis of p38 gamma (MAPK12) Antibody (Center) (Cat.# AP7224c) in mouse lung tissue lysates (35ug/lane). MAPK12 (arrow) was detected using the purified Pab.
  • IHC-P - MAPK12 Antibody (Center) AP7224C
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • WB - MAPK12 Antibody (Center) AP7224C
    Western blot analysis of MAPK12 (arrow) using rabbit polyclonal hMAPK12-P354 (Cat. #AP7224c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MAPK12 gene.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P53778
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 41940 Da
Antigen Region 339-367 aa
Additional Information
Gene ID 6300
Other Names Mitogen-activated protein kinase 12, MAP kinase 12, MAPK 12, Extracellular signal-regulated kinase 6, ERK-6, Mitogen-activated protein kinase p38 gamma, MAP kinase p38 gamma, Stress-activated protein kinase 3, MAPK12, ERK6, SAPK3
Target/Specificity This MAPK12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 339-367 amino acids from the Central region of human MAPK12.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMAPK12 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MAPK12
Synonyms ERK6, SAPK3
Function Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK12 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases such as MAPKAPK2, which are activated through phosphorylation and further phosphorylate additional targets. Plays a role in myoblast differentiation and also in the down-regulation of cyclin D1 in response to hypoxia in adrenal cells suggesting MAPK12 may inhibit cell proliferation while promoting differentiation. Phosphorylates DLG1. Following osmotic shock, MAPK12 in the cell nucleus increases its association with nuclear DLG1, thereby causing dissociation of DLG1-SFPQ complexes. This function is independent of its catalytic activity and could affect mRNA processing and/or gene transcription to aid cell adaptation to osmolarity changes in the environment. Regulates UV-induced checkpoint signaling and repair of UV-induced DNA damage and G2 arrest after gamma- radiation exposure. MAPK12 is involved in the regulation of SLC2A1 expression and basal glucose uptake in L6 myotubes; and negatively regulates SLC2A4 expression and contraction-mediated glucose uptake in adult skeletal muscle. C-Jun (JUN) phosphorylation is stimulated by MAPK14 and inhibited by MAPK12, leading to a distinct AP-1 regulation. MAPK12 is required for the normal kinetochore localization of PLK1, prevents chromosomal instability and supports mitotic cell viability. MAPK12-signaling is also positively regulating the expansion of transient amplifying myogenic precursor cells during muscle growth and regeneration.
Cellular Location Cytoplasm. Nucleus. Mitochondrion. Note=Mitochondrial when associated with SH3BP5. In skeletal muscle colocalizes with SNTA1 at the neuromuscular junction and throughout the sarcolemma (By similarity).
Tissue Location Highly expressed in skeletal muscle and heart.
Research Areas
Citations (0)

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Activation of members of the mitogen-activated protein kinase family is a major mechanism for transduction of extracellular signals. Stress-activated protein kinases are one subclass of MAP kinases. MAPK12 functions as a signal transducer during differentiation of myoblasts to myotubes.


Garcia-Lora, A., et al., Cancer Immunol. Immunother. 52(1):59-64 (2003).
Julien, C., et al., J. Biol. Chem. 278(43):42615-42624 (2003).
Robinson, M.J., et al., J. Biol. Chem. 277(7):5094-5100 (2002).
Court, N.W., et al., J. Mol. Cell. Cardiol. 34(4):413-426 (2002).
Wang, X., et al., Mol. Cell. Biol. 20(13):4543-4552 (2000).

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$ 295.00
$ 99.00
Cat# AP7224C
(40 western blots)
Availability: In Stock
Bulk Size
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