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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   PHKG2 Antibody (N-term)   

PHKG2 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PHKG2 Antibody (N-term) AP7233a
    The anti-PHKG2 Pab (Cat. #AP7233a) is used in Western blot to detect PHKG2 in mouse kidney tissue lysate.
  • WB - PHKG2 Antibody (N-term) AP7233a
    Western blot analysis of PHKG2 (arrow) using PHKG2 Antibody (N-term) (Cat.#AP7233a).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PHKG2 gene (Lane 2) (Origene Technologies).
  • IHC-P - PHKG2 Antibody (N-term) AP7233a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • WB - PHKG2 Antibody (N-term) AP7233a
    PHKG2 Antibody (E56) (Cat. #AP7233a) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the PHKG2 antibody detected the PHKG2 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P15735
Other Accession Q2KJ16
Reactivity Human, Mouse
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 46442 Da
Antigen Region 41-71 aa
Additional Information
Gene ID 5261
Other Names Phosphorylase b kinase gamma catalytic chain, liver/testis isoform, PHK-gamma-LT, PHK-gamma-T, PSK-C3, Phosphorylase kinase subunit gamma-2, PHKG2
Target/Specificity This PHKG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-71 amino acids from the N-terminal region of human PHKG2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPHKG2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PHKG2
Function Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. May regulate glycogeneolysis in the testis. In vitro, phosphorylates PYGM (By similarity).
Research Areas
Citations (0)

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Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway. The calcium/calmodulin-dependent kinase (CAMK) group consists of 75 kinases regulated by Ca2+/CaM and close relative family (CAMK, CAMKL, DAPK, MAPKAPK).


Burwinkel, B., et al., Hum. Mol. Genet. 7(1):149-154 (1998).
Maichele, A.J., et al., Nat. Genet. 14(3):337-340 (1996).
Whitmore, S.A., et al., Genomics 20(2):169-175 (1994).
Hanks, S.K., Mol. Endocrinol. 3(1):110-116 (1989).
Hanks, S.K., Proc. Natl. Acad. Sci. U.S.A. 84(2):388-392 (1987).

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Cat# AP7233a
(40 western blots)
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