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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   SPHK1 Antibody (N-term)   

SPHK1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - SPHK1 Antibody (N-term) AP7237a
    Western blot analysis of SPHK1-M1 Antibody (arrow) using rabbit polyclonal SPHK1-M1 Antibody (Cat. #AP7237a). 293T cell lysates either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPHK1-M1 gene. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody
  • WB - SPHK1 Antibody (N-term) AP7237a
    Western blot analysis of lysate from A431 cell line, using SPHK1 Antibody (M1)(Cat. #AP7237a). AP7237a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
  • WB - SPHK1 Antibody (N-term) AP7237a
    The anti-SphK1 Pab (Cat. #AP7237a) is used in Western blot (Lane 2) to detect c-myc-tagged SphK1 in transfected 293 cell lysate (a c-myc antibody is used as control in Lane 1). Data is kindly provided by Dr. J. Van Brocklyn from the Ohio State University (Columbus, OH).
  • WB - SPHK1 Antibody (N-term) AP7237a
    Western blot analysis of anti-hSPHK1-M1 Pab (Cat. #AP7237a) in 293 cell line lysates transiently transfected with the SPHK1 gene (2ug/lane). hSPHK1-M1(arrow) was detected using the purified Pab.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9NYA1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 42518 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 8877
Other Names Sphingosine kinase 1, SK 1, SPK 1, SPHK1, SPHK, SPK
Target/Specificity This SPHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human SPHK1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSPHK1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SPHK1
Synonyms SPHK, SPK
Function Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.
Cellular Location Cytoplasm. Nucleus. Cell membrane. Note=Translocated from the cytoplasm to the plasma membrane in a CIB1-dependent manner
Tissue Location Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle
Research Areas
Citations ( 0 )


Sphingosine Kinase (SphK) catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. SphK is an important signaling enzyme which is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.


Ota, T., et al., Nat. Genet. 36(1):40-45 (2004).
Nava, V.E., et al., FEBS Lett. 473(1):81-84 (2000).
Melendez, A.J., et al., Gene 251(1):19-26 (2000).
Pitson, S.M., et al., Biochem. J. 350 Pt 2, 429-441 (2000).

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$ 295.00
$ 99.00
Cat# AP7237a
(40 western blots)
Availability: In Stock
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