|Application ||WB, IHC-P, FC, E|
|Calculated MW||42518 Da|
|Antigen Region||313-343 aa|
|Other Names||Sphingosine kinase 1, SK 1, SPK 1, SPHK1, SPHK, SPK|
|Target/Specificity||This SPHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 313-343 amino acids from the C-terminal region of human SPHK1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SPHK1 Antibody (C-term R414) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.|
|Cellular Location||Cytoplasm. Nucleus. Cell membrane. Note=Translocated from the cytoplasm to the plasma membrane in a CIB1-dependent manner|
|Tissue Location||Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle|
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Provided below are standard protocols that you may find useful for product applications.
SPHK1 is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1 (MIM 601974). Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor (e.g., MIM 173430) and serum.
Hengst, J.A., et al. Arch. Biochem. Biophys. 494(1):23-31(2010)
Jarman, K.E., et al. J. Biol. Chem. 285(1):483-492(2010)
Gamble, J.R., et al. Am. J. Pathol. 175(5):2217-2225(2009)
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