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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   SPHK1 Antibody (C-term R414)   

SPHK1 Antibody (C-term R414)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - SPHK1 Antibody (C-term R414) AP7237B
    Western blot analysis of SPHK1 Antibody (C-term R414) (Cat. #AP7237b) in 293 cell line lysates (35ug/lane).SPHK1 (arrow) was detected using the purified Pab.
  • IHC-P - SPHK1 Antibody (C-term R414) AP7237B
    SPHK1 Antibody (C-term R414)(Cat. #AP7237b) IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPHK1 Antibody (C-term R414) for immunohistochemistry. Clinical relevance has not been evaluated.
  • WB - SPHK1 Antibody (C-term R414) AP7237B
    Western blot analysis of SPHK1 (arrow) using rabbit polyclonal SPHK1 Antibody (C-term R414) (Cat. #AP7237b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPHK1 gene.
  • FC - SPHK1 Antibody (C-term R414) AP7237B
    SPHK1 Antibody (C-term R414) (Cat. #AP7237b) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9NYA1
Other Accession Q8N632
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 42518 Da
Antigen Region 313-343 aa
Additional Information
Gene ID 8877
Other Names Sphingosine kinase 1, SK 1, SPK 1, SPHK1, SPHK, SPK
Target/Specificity This SPHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 313-343 amino acids from the C-terminal region of human SPHK1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSPHK1 Antibody (C-term R414) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SPHK1
Synonyms SPHK, SPK
Function Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.
Cellular Location Cytoplasm. Nucleus. Cell membrane. Note=Translocated from the cytoplasm to the plasma membrane in a CIB1-dependent manner
Tissue Location Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle
Research Areas
Citations (0)

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SPHK1 is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1 (MIM 601974). Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor (e.g., MIM 173430) and serum.


Hengst, J.A., et al. Arch. Biochem. Biophys. 494(1):23-31(2010)
Jarman, K.E., et al. J. Biol. Chem. 285(1):483-492(2010)
Gamble, J.R., et al. Am. J. Pathol. 175(5):2217-2225(2009)

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Cat# AP7237B
(40 western blots)
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