- CITATIONS: 8
|Application ||WB, IHC-P, E|
|Calculated MW||69217 Da|
|Antigen Region||1-30 aa|
|Other Names||Sphingosine kinase 2, SK 2, SPK 2, SPHK2|
|Target/Specificity||This SPHK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human SPHK2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Precautions||SPHK2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro- dihydrosphingosine, D-erythro-sphingosine and L-threo- dihydrosphingosine. Binds phosphoinositides.|
|Cellular Location||Isoform 1: Cytoplasm. Membrane.|
Provided below are standard protocols that you may find useful for product applications.
Sphingosine Kinase (SphK) catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. SphK is an important signaling enzyme which is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.
Shu, X., et al., Mol. Cell. Biol. 22(22):7758-7768 (2002).
Xia, P., et al., J. Biol. Chem. 277(10):7996-8003 (2002).
Liu, H., et al., J. Biol. Chem. 275(26):19513-19520 (2000).
Shutler, G., et al., Genomics 34(3):334-339 (1996).
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