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>   home   >   Products   >   Primary Antibodies   >   EIF4A1 Antibody (N-term)   

EIF4A1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - EIF4A1 Antibody (N-term) AP7262a
    Western blot analysis of anti-EIF4A1 Pab (Cat. #AP7262a) in K562 cell line lysates (35ug/lane). EIF4A1(arrow) was detected using the purified Pab.
  • WB - EIF4A1 Antibody (N-term) AP7262a
    Western blot analysis of EIF4A1 (arrow) using rabbit polyclonal EIF4A1 Antibody (N-term) (Cat.#AP7262a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF4A1 gene (Lane 2) (Origene Technologies).
  • IHC-P - EIF4A1 Antibody (N-term) AP7262a
    Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with EIF4A1 antibody (N-term) (Cat.#AP7262a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P60842
Other Accession P60843, Q4R8K5, Q3SZ54
Reactivity Human
Predicted Bovine, Monkey, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 46154 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 1973
Other Names Eukaryotic initiation factor 4A-I, eIF-4A-I, eIF4A-I, ATP-dependent RNA helicase eIF4A-1, EIF4A1, DDX2A, EIF4A
Target/Specificity This EIF4A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human EIF4A1.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEIF4A1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EIF4A1
Synonyms DDX2A, EIF4A
Function ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
Citations (0)

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Background

EIF4A1 is an ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

References

Suzuki,C., Proc. Natl. Acad. Sci. U.S.A. 105 (9), 3274-3279 (2008) Kim,W.J., EMBO J. 26 (24), 5020-5032 (2007)

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$ 295.00
$ 99.00
Cat# AP7262a
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(40 western blots)
Availability: In Stock
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