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ADRB2 Antibody (S364)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Country
United States
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Ordering Information
Catalog # Size Availability Price  
AP7263e 0.1 mg 400 ul In Stock $ 255.00 Add to cart
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ADRB2 Antibody (S364) - Product info

ApplicationWB, IHC, FC
  • Applications Legend:
  • W=Western Blotting
  • IP=Immunoprecipitation
  • IHC-P=Immunohistochemistry (Paraffin)
  • IF-IC=Immunofluorescence (Immunocytochemistry)
  • F=Flow Cytometry
Primary AccessionP07550
ReactivityHuman
Concentration0.25 mg/ml
IsotypeRabbit Ig
Calculated MW46459 Da

ADRB2 Antibody (S364) - Additional info

Gene ID 154
Other Names
ADRB2; ADRB2R; B2AR; Beta-2 adrenergic receptor; Beta-2 adrenoreceptor
Target/Specificity
This ADRB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 352~381 amino acids surrounding S364 of human BAR2.
Dilution
WB~~1:50~100
IHC~~1:50~100
FC~~1:10~50
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions
ADRB2 Antibody (S364) is for research use only and not for use in diagnostic or therapeutic procedures.

ADRB2 Antibody (S364) - Protein Information

Name ADRB2
Synonyms ADRB2R, B2AR
Function
Beta-adrenergic receptors mediate the catecholamine- induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine
Cellular Location
Cell membrane; Multi-pass membrane protein. Note=Colocalizes with VHL at the cell membrane

ADRB2 Antibody (S364) - Related products

AP3548a: Phospho-BAR2-pS261 Antibody

AP3598a: Phospho-BAR2-pS364 Antibody

AP3634a: Phospho-BAR2-pT384 Antibody

AP7263a: beta 2 Adrenergic Receptor (BAR2) Antibody (N-term)

AP7263d: BAR2 Antibody (S261)

AP7263e: ADRB2 Antibody (S364)

AP7263f: ADRB2 Antibody (T384)

RI10153: ADRB2 predesign siRNA

DC08360: Human ADRB2 cDNA Clone

BP3548a: Phospho-BAR2-pS261 Antibody Blocking Peptide

BP3598a: Phospho-BAR2-pS364 Antibody Blocking Peptide

BP3634a: Phospho-BAR2-pT384 Antibody Blocking Peptide

BP7263a: BAR2 Antibody (N-term) Blocking Peptide

BP7263d: BAR2 Antibody (S261) Blocking Peptide

BP7263e: ADRB2 Antibody (S364) Blocking Peptide

BP7263f: ADRB2 Antibody (T384) Blocking Peptide

AF1033a: ADRB2R / ADRB2 Antibody

ADRB2 Antibody (S364) - Application data

  • Western blot analysis of anti-ADRB2 Antibody (S364)(Cat.#AP7263e) in NCI-H460 cell line lysates (35ug/lane). ADRB2 (arrow) was detected using the purified Pab.

  • Formalin-fixed and paraffin-embedded human brain reacted with ADRB2 Antibody (S364), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

  • Flow cytometric analysis of NCI-H292 cells using ADRB2 Antibody (S364)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

ADRB2 Antibody (S364) - Research Areas

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Provided below are standard protocols that you may find useful for product applications.

BACKGROUND

ADRB2, beta-2-adrenergic receptor which is a member of the G protein-coupled receptor superfamily. This receptor is directly associated with one of its ultimate effectors, the class C L-type calcium channel Ca(V)1.2. This receptor-channel complex also contains a G protein, an adenylyl cyclase, cAMP-dependent kinase, and the counterbalancing phosphatase, PP2A. The assembly of the signaling complex provides a mechanism that ensures specific and rapid signaling by this G protein-coupled receptor. This protein is intronless.

REFERENCES

Kobilka B.K., Dixon R.A.F.Proc. Natl. Acad. Sci. U.S.A. 84:46-50(1987) Emorine L.J., Marullo S.Proc. Natl. Acad. Sci. U.S.A. 84:6995-6999(1987) Kobilka B.K., Frielle T.J. Biol. Chem. 262:7321-7327(1987)