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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   CASC3 Antibody (Y181)   

CASC3 Antibody (Y181)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - CASC3 Antibody (Y181) AP7264d
    Anti-CASC3 Antibody (Y181) at 1:1000 dilution + NCI-H460 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 76 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - CASC3 Antibody (Y181) AP7264d
    Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with CASC3 Antibody (Y181) (Cat.#AP7264d), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O15234
Other Accession A0JMU8, Q8K3X0, Q8K3W3, Q1ECZ4, A5D7H5, NP_619601
Reactivity Human
Predicted Bovine, Zebrafish, Mouse, Rat, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 76278 Da
Antigen Region 159-188 aa
Additional Information
Gene ID 22794
Other Names Protein CASC3, Cancer susceptibility candidate gene 3 protein, Metastatic lymph node gene 51 protein, MLN 51, Protein barentsz, Btz, CASC3, MLN51
Target/Specificity This CASC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-188 amino acids from human CASC3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCASC3 Antibody (Y181) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CASC3
Synonyms MLN51
Function Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Stimulates the ATPase and RNA- helicase activities of EIF4A3. Plays a role in the stress response by participating in cytoplasmic stress granules assembly and by favoring cell recovery following stress. Component of the dendritic ribonucleoprotein particles (RNPs) in hippocampal neurons. May play a role in mRNA transport. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Binds poly(G) and poly(U) RNA homopolymer.
Cellular Location Cytoplasm, perinuclear region. Nucleus. Nucleus speckle. Note=Predominantly found in the perinuclear region (By similarity). Shuttles between the nucleus and the cytoplasm in a CRM1-dependent manner. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Shuttles between the cytoplasm and the stress granules. More specifically found in nuclear intrachromatin granules clusters (IGC), also called nuclear speckles, which are storage compartments for nuclear proteins involved in mRNA processing. Colocalizes in nuclear speckles with MAGOH. Under stress condition, colocalizes with FMR1 and TIA1, but not MAGOH and RBM8A EJC core factors, in cytoplasmic stress granules, which contain stored mRNAs whose translation is stopped in response to stress.
Tissue Location Widely expressed. Overexpressed in breast cancers and metastasis, as well as in gastric cancers
Research Areas
Citations (0)

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Component of the dendritic ribonucleoprotein particles (RNPs) in hippocampal neurons. May play a role in mRNA transport.


Macchi,P., J. Neurosci. 23 (13), 5778-5788 (2003)
Degot,S., Oncogene 21 (28), 4422-4434 (2002)

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$ 295.00
$ 99.00
Cat# AP7264d
(40 western blots)
Availability: In Stock
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