|Application ||WB, IHC-P, E|
|Other Accession||Q9Z1A5, Q8VBW6, Q4R3L6, NP_003896|
|Predicted||Monkey, Mouse, Rat|
|Calculated MW||60246 Da|
|Antigen Region||430-459 aa|
|Other Names||NEDD8-activating enzyme E1 regulatory subunit, Amyloid beta precursor protein-binding protein 1, 59 kDa, APP-BP1, Amyloid protein-binding protein 1, Proto-oncogene protein 1, NAE1, APPBP1|
|Target/Specificity||This NAE1 (APPBP1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 430-459 amino acids from the C-terminal region of human NAE1 (APPBP1).|
|Format||Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||NAE1 (APPBP1) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.|
|Cellular Location||Cell membrane. Note=Colocalizes with APP in lipid rafts|
|Tissue Location||Ubiquitous in fetal tissues. Expressed throughout the adult brain.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
APPBP1 binds to the beta-amyloid precursor protein, a cell surface protein with signal-transducing properties thought to play a role in the pathogenesis of Alzheimer's disease. In addition, this protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. APPB1 is required for cell cycle progression through the S/M checkpoint.
Chen,Y., J. Cell Biol. 163 (1), 27-33 (2003)
Chen,Y., J. Neurochem. 85 (3), 801-809 (2003)
Walden,H., Nature 422 (6929), 330-334 (2003)
Chow,N., J. Biol. Chem. 271 (19), 11339-11346 (1996)
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