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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   INS (Insulin) Antibody (Center)   

INS (Insulin) Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - INS (Insulin) Antibody (Center) AP7277c
    INS (Insulin) Antibody (Center) (Cat. #AP7277c) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the INS antibody detected the INS protein (arrow).
  • IF - INS (Insulin) Antibody (Center) AP7277c
    Confocal immunofluorescent analysis of INS (Insulin) Antibody (Center) (Cat. #AP7277c) with pancreas tissue followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
  • IHC-P - INS (Insulin) Antibody (Center) AP7277c
    INS Antibody (Insulin) (Center) (Cat. #AP7277c)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of INS Antibody (Insulin) (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - INS (Insulin) Antibody (Center) AP7277c
    INS (Insulin) Antibody (Center) (Cat. #AP7277c) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, FC, E
Primary Accession P01308
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 11981 Da
Antigen Region 35-64 aa
Additional Information
Gene ID 3630
Other Names Insulin, Insulin B chain, Insulin A chain, INS
Target/Specificity This INS (Insulin) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 35-64 amino acids from the Central region of human INS (Insulin).
Dilution WB~~1:1000
IF~~1:10~50
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsINS (Insulin) Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name INS
Function Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
Cellular Location Secreted.
Research Areas
Citations (0)

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Background

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake.

References

Nordquist,L., Diabetes Metab. Res. Rev. 24 (2), 165-168 (2008) Nordquist,L., (er) Am. J. Physiol. Regul. Integr. Comp. Physiol. (2007) In press Naya,T., Angiology 58 (6), 677-684 (2007)

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Discontinued
Cat# AP7277c
Size:
(40 western blots)
Alternative Products:

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