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Parg Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
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- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | Q86W56 |
| Other Accession | O88622 |
| Reactivity | Human |
| Predicted | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 111110 Da |
| Antigen Region | 390-421 aa |
| Gene ID | 670;8505 |
|---|---|
| Other Names | Poly(ADP-ribose) glycohydrolase, PARG |
| Target/Specificity | This Parg antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 390-421 amino acids from the C-terminal region of human Parg. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Parg Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PARG {ECO:0000303|PubMed:14527731, ECO:0000312|HGNC:HGNC:8605} |
|---|---|
| Function | Poly(ADP-ribose) glycohydrolase that degrades poly(ADP- ribose) by hydrolyzing the ribose-ribose bonds present in poly(ADP- ribose) (PubMed:15450800, PubMed:21892188, PubMed:23102699, PubMed:23474714, PubMed:33186521, PubMed:34321462, PubMed:34019811). PARG acts both as an endo- and exoglycosidase, releasing poly(ADP- ribose) of different length as well as ADP-ribose monomers (PubMed:23102699, PubMed:23481255). It is however unable to cleave the ester bond between the terminal ADP-ribose and ADP-ribosylated residues, leaving proteins that are mono-ADP-ribosylated (PubMed:21892188, PubMed:23474714, PubMed:33186521). Poly(ADP-ribose) is synthesized after DNA damage is only present transiently and is rapidly degraded by PARG (PubMed:23102699, PubMed:34019811). Required to prevent detrimental accumulation of poly(ADP-ribose) upon prolonged replicative stress, while it is not required for recovery from transient replicative stress (PubMed:24906880). Responsible for the prevalence of mono-ADP-ribosylated proteins in cells, thanks to its ability to degrade poly(ADP-ribose) without cleaving the terminal protein-ribose bond (PubMed:33186521). Required for retinoid acid- dependent gene transactivation, probably by removing poly(ADP-ribose) from histone demethylase KDM4D, allowing chromatin derepression at RAR- dependent gene promoters (PubMed:23102699). Involved in the synthesis of ATP in the nucleus, together with PARP1, NMNAT1 and NUDT5 (PubMed:27257257). Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (PubMed:27257257). |
| Cellular Location | [Isoform 1]: Nucleus Note=Colocalizes with PCNA at replication foci (PubMed:21398629) Relocalizes to the cytoplasm in response to DNA damage (PubMed:16460818). [Isoform 3]: Cytoplasm [Isoform 5]: Mitochondrion matrix |
| Tissue Location | Ubiquitously expressed. |
Research Areas
Citations (0)
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
Poly(ADP-ribose) glycohydrolase (PARG) is the major enzyme responsible for the catabolism of poly(ADP-ribose), a reversible covalent-modifier of chromosomal proteins. The protein is found in many tissues and may be subject to proteolysis generating smaller, active products.
References
Meyer,R.G., Exp. Cell Res. 313 (13), 2920-2936 (2007)
Fisher,A.E., Mol. Cell. Biol. 27 (15), 5597-5605 (2007)
Keil,C., J. Biol. Chem. 281 (45), 34394-34405 (2006)
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$ 365.00
$ 140.00
Cat# AP7283B
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