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JLP (SPAG9) Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - JLP (SPAG9) Antibody (Center) AP7287c
    Western blot analysis of SPAG9 (arrow) using rabbit polyclonal SPAG9 Antibody (Center) (Cat.#AP7287c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SPAG9 gene (Lane 2) (Origene Technologies).
  • IHC-P - JLP (SPAG9) Antibody (Center) AP7287c
    Formalin-fixed and paraffin-embedded human testis tissue reacted with SPAG9 antibody (Center) (Cat.#AP7287c), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O60271
Other Accession Q58A65
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 146205 Da
Antigen Region 603-632 aa
Additional Information
Gene ID 9043
Other Names C-Jun-amino-terminal kinase-interacting protein 4, JIP-4, JNK-interacting protein 4, Cancer/testis antigen 89, CT89, Human lung cancer oncogene 6 protein, HLC-6, JNK-associated leucine-zipper protein, JLP, Mitogen-activated protein kinase 8-interacting protein 4, Proliferation-inducing protein 6, Protein highly expressed in testis, PHET, Sperm surface protein, Sperm-associated antigen 9, Sperm-specific protein, Sunday driver 1, SPAG9, HSS, KIAA0516, MAPK8IP4, SYD1
Target/Specificity This JLP (SPAG9) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 603-632 amino acids from the Central region of human JLP (SPAG9).
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsJLP (SPAG9) Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SPAG9
Synonyms HSS, KIAA0516, MAPK8IP4, SYD1
Function The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Isoform 5 may play a role in spermatozoa-egg- interaction.
Cellular Location Cytoplasm. Cytoplasm, perinuclear region. Note=Perinuclear distribution in response to stress signals such as UV radiation
Tissue Location Isoform 5 is expressed only in testis on the round spermatids of stage I, II and II. Isoform 5 is absent in spermatogonia and spermatocyte. Isoform 3 is expressed in testis Isoform 4 is expressed in testis and in acute myeloid leukemia (AML) patients.
Research Areas
Citations (0)

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SPAG9, which is abundantly expressed in testicular haploid germ cells, is recognized by sperm-agglutinating antibodies and implicated in infertility.


Rana,R., Hum. Reprod. 21 (11), 2894-2900 (2006)
Rana,R., Biochem. Biophys. Res. Commun. 340 (1), 158-164 (2006)
Jagadish,N., Keio J Med 54 (2), 66-71 (2005)

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Cat# AP7287c
(40 western blots)
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