|Application ||WB, IHC-P, E|
|Calculated MW||48551 Da|
|Antigen Region||358-389 aa|
|Other Names||Cyclin-A2, Cyclin-A, CCNA2, CCN1, CCNA|
|Target/Specificity||This CCNA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 358-389 amino acids from the C-terminal region of human CCNA2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CCNA2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Cyclin which controls both the G1/S and the G2/M transition phases of the cell cycle. Functions through the formation of specific serine/threonine protein kinase holoenzyme complexes with the cyclin-dependent protein kinases CDK1 or CDK2. The cyclin subunit confers the substrate specificity of these complexes and differentially interacts with and activates CDK1 and CDK2 throughout the cell cycle.|
|Cellular Location||Nucleus. Cytoplasm Note=Exclusively nuclear during interphase (PubMed:1312467) Detected in the nucleus and the cytoplasm at prophase (PubMed:1312467). Cytoplasmic when associated with SCAPER (PubMed:17698606).|
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Provided below are standard protocols that you may find useful for product applications.
CCNA2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.
Henglein B.,Proc. Natl. Acad. Sci. U.S.A. 91:5490-5494(1994)
Wang J.,Nature 343:555-557(1990)
Tsang,W.Y.,J. Cell Biol. 178 (4), 621-633 (2007)
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