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LAP3 Antibody (N-term)Purified Rabbit Polyclonal Antibody (Pab)

Country
United States
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Ordering Information
Catalog # Size Availability Price  
AP7296a 0.1 mg 400 ul In Stock $ 255.00 Add to cart
  • Specification
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  • Reviews
  • Protocols
  • Backgrounds

LAP3 Antibody (N-term) - Product info

ApplicationWB, IHC
  • Applications Legend:
  • W=Western Blotting
  • IP=Immunoprecipitation
  • IHC-P=Immunohistochemistry (Paraffin)
  • IF-IC=Immunofluorescence (Immunocytochemistry)
  • F=Flow Cytometry
Primary AccessionP28838
ReactivityHuman
Concentration0.25 mg/ml
IsotypeRabbit Ig
Calculated MW56166 Da

LAP3 Antibody (N-term) - Additional info

Gene ID 51056
Other Names
LAP3; LAPEP; PEPS; Cytosol aminopeptidase; Leucine aminopeptidase 3; Leucyl aminopeptidase; Peptidase S; Proline aminopeptidase; Prolyl aminopeptidase
Target/Specificity
This LAP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 97~126 amino acids from the N-term of human LAP3.
Dilution
WB~~1:50~100
IHC~~1:10~50
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions
LAP3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

LAP3 Antibody (N-term) - Protein Information

Name LAP3
Synonyms LAPEP, PEPS
Function
Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides
Cellular Location
Cytoplasm.

LAP3 Antibody (N-term) - Related products

AP7296a: LAP3 Antibody (N-term)

AP7296b: LAP3 Antibody (C-term)

RI12981: LAP3 predesign siRNA

DC08514: Human LAP3 cDNA Clone

LY11987a: LAP3 Over-expression Lysate

BP7296a: LAP3 Antibody (N-term) Blocking Peptide

BP7296b: LAP3 Antibody (C-term) Blocking Peptide

LAP3 Antibody (N-term) - Application data

  • Western blot analysis of anti-LAP3(N-term) Pab (Cat.#AP7296a) in HepG2 cell line lysates (35ug/lane). LAP3(arrow) was detected using the purified Pab (1:60 dilution).

  • Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with LAP3 antibody (N-term) (Cat.#AP7296a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

LAP3 Antibody (N-term) - Research Areas

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Provided below are standard protocols that you may find useful for product applications.

BACKGROUND

LAP3 is presumably involved in the processing and regular turnover of intracellular proteins. It catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro.

REFERENCES

Goto,Y.,FEBS Lett. 580 (7), 1833-1838 (2006) Matsushima,M.,Biochem. Biophys. Res. Commun. 178 (3), 1459-1464 (1991)