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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Lefty (LEFTB) Antibody (C-term)   

Lefty (LEFTB) Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Lefty (LEFTB) Antibody (C-term) AP7297b
    Western blot analysis of anti-LEFTB(C-term) Pab (Cat.#AP7297b) in HL60 cell line lysates (35ug/lane). LEFTB(arrow) was detected using the purified Pab.
  • IHC-P - Lefty (LEFTB) Antibody (C-term) AP7297b
    Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with LEFTB antibody (C-term) (Cat.#AP7279b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession O75610
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 40880 Da
Antigen Region 283-314 aa
Additional Information
Gene ID 10637
Other Names Left-right determination factor 1, Left-right determination factor B, Protein lefty-1, Protein lefty-B, LEFTY1, LEFTB, LEFTYB
Target/Specificity This Lefty (LEFTB) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 283-314 amino acids from the C-terminal region of human Lefty (LEFTB).
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLefty (LEFTB) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LEFTY1
Synonyms LEFTB, LEFTYB
Function Required for left-right axis determination as a regulator of LEFTY2 and NODAL.
Cellular Location Secreted.
Research Areas
Citations (0)

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Background

LEFTB is a member of the TGF-beta family of proteins. A similar secreted protein in mouse plays a role in left-right asymmetry determination of organ systems during development. Alternative processing of this protein can yield three different products.

References

Kosaki K.,Am. J. Hum. Genet. 64:712-721(1999)
Dvash,T.,Stem Cells 25 (2), 465-472 (2007)
Besser,D.,J. Biol. Chem. 279 (43), 45076-45084 (2004)

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$ 295.00
$ 99.00
Cat# AP7297b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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