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CA5B Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CA5B Antibody (C-term) AP7308b
    Western blot analysis of CA5B Antibody (C-term) (Cat. #AP7308b) in mouse liver tissue lysates (35ug/lane). CA5B (arrow) was detected using the purified Pab.
    detail
  • IHC-P - CA5B Antibody (C-term) AP7308b
    CA5B Antibody (C-term) (Cat. #AP7308b) IHC analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CA5B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - CA5B Antibody (C-term) AP7308b
    CA5B Antibody (C-term) (Cat. #AP7308b) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession Q9Y2D0
Other Accession Q66HG6, Q9QZA0
Reactivity Human, Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 36434 Da
Antigen Region 253-283 aa
Additional Information
Gene ID 11238
Other Names Carbonic anhydrase 5B, mitochondrial, Carbonate dehydratase VB, Carbonic anhydrase VB, CA-VB, CA5B
Target/Specificity This CA5B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-283 amino acids from the C-terminal region of human CA5B.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCA5B Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CA5B
Function Mitochondrial carbonic anhydrase that catalyzes the reversible conversion of carbon dioxide to bicarbonate/HCO3.
Cellular Location Mitochondrion.
Tissue Location Strongest expression in heart, pancreas, kidney, placenta, lung, and skeletal muscle. Not expressed in liver
Research Areas
Citations (0)
citation

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Background

CA5B belongs a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. This protein is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA, CA VA. The protein has a wider tissue distribution than CA VA, which is restricted to the liver.

References

Vullo,D., Nishimori,I. Bioorg. Med. Chem. Lett. 17 (5), 1336-1340 (2007)
Shah,G.N. Proc. Natl. Acad. Sci. U.S.A. 97 (4), 1677-1682 (2000)
Fujikawa-Adachi,K. J. Biol. Chem. 274 (30), 21228-21233 (1999)

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$ 365.00
$ 140.00
Cat# AP7308b
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