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POLE3 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - POLE3 Antibody (N-term) AP7316a
    POLE3 Antibody (N-term) (Cat. #AP7316a) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the POLE3 antibody detected the POLE3 protein (arrow).
  • FC - POLE3 Antibody (N-term) AP7316a
    POLE3 Antibody (N-term) (Cat. #AP7316a) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9NRF9
Other Accession Q642A5, Q9JKP7, Q3SZN5
Reactivity Human
Predicted Bovine, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 16860 Da
Antigen Region 5-34 aa
Additional Information
Gene ID 54107
Other Names DNA polymerase epsilon subunit 3, Arsenic-transactivated protein, AsTP, Chromatin accessibility complex 17 kDa protein, CHRAC-17, HuCHRAC17, DNA polymerase II subunit 3, DNA polymerase epsilon subunit p17, POLE3, CHRAC17
Target/Specificity This POLE3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5-34 amino acids from the N-terminal region of human POLE3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPOLE3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name POLE3
Synonyms CHRAC17
Function Forms a complex with DNA polymerase epsilon subunit CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1.
Cellular Location Nucleus.
Tissue Location Expressed in all tissues tested, including, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas
Research Areas
Citations (0)

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POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.


Bolognese,F., Imbriano,C. Nucleic Acids Res. 28 (19), 3830-3838 (2000)
Li,Y., Pursell,Z.F. J. Biol. Chem. 275 (30), 23247-23252 (2000)

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Cat# AP7316a
(40 western blots)
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