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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   MFAP5 Antibody (C-term)   

MFAP5 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - MFAP5 Antibody (C-term) AP7319b
    Western blot analysis of MFAP5 antibody (C-term) (Cat.#AP7319b) in K562 cell line lysates (35ug/lane). MFAP5 (arrow) was detected using the purified Pab.
    detail
  • WB - MFAP5 Antibody (C-term) AP7319b
    Western blot analysis of MFAP5 (arrow) using rabbit polyclonal MFAP5 Antibody (C-term) (Cat. #AP7319b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MFAP5 gene.
    detail
  • IHC-P - MFAP5 Antibody (C-term) AP7319b
    Formalin-fixed and paraffin-embedded human Skeletal muscle reacted with MFAP5 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - MFAP5 Antibody (C-term) AP7319b
    MFAP5 Antibody (C-term) (Cat. #AP7319b) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, IHC-P, E
Primary Accession Q13361
Other Accession Q9QZJ6, Q28022
Reactivity Human
Predicted Bovine, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 19612 Da
Antigen Region 133-160 aa
Additional Information
Gene ID 8076
Other Names Microfibrillar-associated protein 5, MFAP-5, MP25, Microfibril-associated glycoprotein 2, MAGP-2, MFAP5, MAGP2
Target/Specificity This MFAP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 133-160 amino acids from the C-terminal region of human MFAP5.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMFAP5 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MFAP5
Synonyms MAGP2
Function May play a role in hematopoiesis. In the cardiovascular system, could regulate growth factors or participate in cell signaling in maintaining large vessel integrity (By similarity). Component of the elastin-associated microfibrils (PubMed:8557636).
Cellular Location Secreted, extracellular space, extracellular matrix
Research Areas
Citations (0)
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Background

MFAP5 is a 25-kD microfibril-associated glycoprotein which is rich in serine and threonine residues. The protein lacks a hydrophobic carboxyl terminus and proline-, glutamine-, and tyrosine-rich regions, which are characteristics of a related 31-kDa microfibril-associated glycoprotein (MFAP2). The close similarity between these two proteins is confined to a central region of 60 aa where precise alignment of 7 cysteine residues occurs. The structural differences suggest that this protein has some functions that are distinct from those of MFAP2.

References

Albig,A.R., Becenti,D.J. Microvasc. Res. 76 (1), 7-14 (2008)
Miyamoto,A., Lau,R. J. Biol. Chem. 281 (15), 10089-10097 (2006)
Penner,A.S., Rock,M.J. J. Biol. Chem. 277 (38), 35044-35049 (2002)
Hatzinikolas,G. J. Biol. Chem. 273 (45), 29309-29314 (1998)

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$ 365.00
$ 140.00
Cat# AP7319b
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