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MSRA Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - MSRA Antibody (N-term) AP7321A
    Anti-MSRA Antibody (N-term) at 1:1000 dilution + human kidney lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IF - MSRA Antibody (N-term) AP7321A
    Immunofluorescent analysis of A549 cells, using MSRA Antibody (N-term) (Cat. #AP7321a). AP7321a was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor® 554 (red) conjugated Phalloidin (red).
  • WB - MSRA Antibody (N-term) AP7321A
    Western blot analysis of MSRA antibody (N-term) (Cat.#AP7321a) in mouse kidney tissue lysates (35ug/lane). MSRA (arrow) was detected using the purified Pab.
  • IHC-P - MSRA Antibody (N-term) AP7321A
    Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MSRA Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - MSRA Antibody (N-term) AP7321A
    MSRA Antibody (N-term) (Cat. #AP7321a) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9UJ68
Other Accession P54149
Reactivity Human, Mouse
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 26132 Da
Antigen Region 34-63 aa
Additional Information
Gene ID 4482
Other Names Mitochondrial peptide methionine sulfoxide reductase, Peptide-methionine (S)-S-oxide reductase, Peptide Met(O) reductase, Protein-methionine-S-oxide reductase, PMSR, MSRA
Target/Specificity This MSRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 34-63 amino acids from the N-terminal region of human MSRA.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMSRA Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine.
Cellular Location Isoform 1: Mitochondrion. Isoform 3: Cytoplasm. Nucleus.
Tissue Location Ubiquitous. Highest expression in adult kidney and cerebellum, followed by liver, heart ventricles, bone marrow and hippocampus
Research Areas
Citations (0)

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MSRA is ubiquitous and highly conserved. This protein carries out the enzymatic reduction of methionine sulfoxide to methionine. Human and animal studies have shown the highest levels of expression in kidney and nervous tissue. The protein's proposed function is the repair of oxidative damage to proteins to restore biological activity.


Pascual,I., Larrayoz,I.M. Genomics 93 (1), 62-71 (2009)
Schallreuter,K.U., Rubsam,K. J. Invest. Dermatol. 128 (4), 808-815 (2008)
Picot,C.R., Perichon,M. FEBS Lett. 558 (1-3), 74-78 (2004)
Vougier,S., Mary,J. Biochem. J. 373 (PT 2), 531-537 (2003)

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$ 295.00
$ 99.00
Cat# AP7321A
(40 western blots)
Availability: In Stock
Bulk Size
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