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GSN Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - GSN Antibody (N-term) AP7326a
    All lanes : Anti-GSN Antibody (N-term) at 1:1000-1:2000 dilution Lane 1: human plasma lysates Lane 2: Hela whole cell lysates Lane 3: mouse spleen lysates Lane 4: rat kidney lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 86 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - GSN Antibody (N-term) AP7326a
    Anti-GSN Antibody (N-term)at 1:2000 dilution + MCF-7 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 86 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - GSN Antibody (N-term) AP7326a
    GSN Antibody (N-term) (RB18721) IHC analysis in formalin fixed and paraffin embedded human Skeletal muscle tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GSN Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - GSN Antibody (N-term) AP7326a
    GSN Antibody (N-term) (Cat. #AP7326a) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, IHC-P, E
Primary Accession P06396
Other Accession Q3SX14
Reactivity Human, Mouse, Rat
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 85698 Da
Antigen Region 230-259 aa
Additional Information
Gene ID 2934
Other Names Gelsolin, AGEL, Actin-depolymerizing factor, ADF, Brevin, GSN
Target/Specificity This GSN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 230-259 amino acids from the N-terminal region of human GSN.
Dilution WB~~1:2000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGSN Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GSN
Function Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed (PubMed:19666512). Plays a role in ciliogenesis (PubMed:20393563).
Cellular Location [Isoform 2]: Cytoplasm, cytoskeleton.
Tissue Location Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells
Research Areas
Citations (0)
citation

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Background

GSN binds to the 'plus' ends of actin monomers and filaments to prevent monomer exchange. The calcium-regulated protein functions in both assembly and disassembly of actin filaments. Defects in this protein are a cause of familial amyloidosis Finnish type (FAF).

References

Li,Q., Ye,Z. Biochem. Biophys. Res. Commun. 385 (2), 284-289 (2009)
Walsh,N., Dowling,P. J Proteomics 71 (5), 561-571 (2008)
Paunio,T., Kiuru,S. Genomics 13 (1), 237-239 (1992)

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$ 182.50
$ 70.00
Cat# AP7326a
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