|Application ||WB, IHC-P, FC, E|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||85698 Da|
|Antigen Region||230-259 aa|
|Other Names||Gelsolin, AGEL, Actin-depolymerizing factor, ADF, Brevin, GSN|
|Target/Specificity||This GSN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 230-259 amino acids from the N-terminal region of human GSN.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||GSN Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.|
|Cellular Location||Isoform 2: Cytoplasm, cytoskeleton.|
|Tissue Location||Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells|
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Provided below are standard protocols that you may find useful for product applications.
GSN binds to the 'plus' ends of actin monomers and filaments to prevent monomer exchange. The calcium-regulated protein functions in both assembly and disassembly of actin filaments. Defects in this protein are a cause of familial amyloidosis Finnish type (FAF).
Li,Q., Ye,Z. Biochem. Biophys. Res. Commun. 385 (2), 284-289 (2009)
Walsh,N., Dowling,P. J Proteomics 71 (5), 561-571 (2008)
Paunio,T., Kiuru,S. Genomics 13 (1), 237-239 (1992)
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