- CITATIONS: 2
|Application ||WB, IHC-P, FC, E|
|Calculated MW||21646 Da|
|Antigen Region||156-182 aa|
|Other Names||Methylated-DNA--protein-cysteine methyltransferase, 6-O-methylguanine-DNA methyltransferase, MGMT, O-6-methylguanine-DNA-alkyltransferase, MGMT|
|Target/Specificity||This MGMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-182 amino acids from the C-terminal region of human MGMT.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MGMT Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.|
Provided below are standard protocols that you may find useful for product applications.
MGMT is involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. This protein repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the protein is irreversibly inactivated.
Kim,J.I., Suh,J.T. Hum. Pathol. 40 (7), 934-941 (2009)
Chen,S.P., Chiu,S.C. Genet Test Mol Biomarkers 13 (1), 67-71 (2009)
Slupphaug,G., Lettrem,I. Carcinogenesis 13 (10), 1769-1773 (1992)
Lee,S.M., Crowther,D. Br. J. Cancer 66 (2), 331-336 (1992)
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