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MGMT Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - MGMT Antibody (C-term) AP7341b
    MGMT Antibody (C-term) (Cat. #AP7341b) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the MGMT antibody detected the MGMT protein (arrow).
  • IHC-P - MGMT Antibody (C-term) AP7341b
    Formalin-fixed and paraffin-embedded human placenta with MGMT Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - MGMT Antibody (C-term) AP7341b
    Flow cytometric analysis of CEM cells using MGMT Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • SPECIFICATION
  • CITATIONS: 2
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession P16455
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 21646 Da
Antigen Region 156-182 aa
Additional Information
Gene ID 4255
Other Names Methylated-DNA--protein-cysteine methyltransferase, 6-O-methylguanine-DNA methyltransferase, MGMT, O-6-methylguanine-DNA-alkyltransferase, MGMT
Target/Specificity This MGMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-182 amino acids from the C-terminal region of human MGMT.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMGMT Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MGMT
Function Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Cellular Location Nucleus.
Research Areas
Citations ( 0 )

Background

MGMT is involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. This protein repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the protein is irreversibly inactivated.

References

Kim,J.I., Suh,J.T. Hum. Pathol. 40 (7), 934-941 (2009)
Chen,S.P., Chiu,S.C. Genet Test Mol Biomarkers 13 (1), 67-71 (2009)
Slupphaug,G., Lettrem,I. Carcinogenesis 13 (10), 1769-1773 (1992)
Lee,S.M., Crowther,D. Br. J. Cancer 66 (2), 331-336 (1992)

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$ 295.00
$ 99.00
Cat# AP7341b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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