MYO1A Antibody (Center S291)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| FC, WB, E |
---|---|
Primary Accession | Q9UBC5 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 118401 Da |
Antigen Region | 276-304 aa |
Gene ID | 4640 |
---|---|
Other Names | Unconventional myosin-Ia, Brush border myosin I, BBM-I, BBMI, Myosin I heavy chain, MIHC, MYO1A, MYHL |
Target/Specificity | This MYO1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276-304 amino acids from the Central region of human MYO1A. |
Dilution | WB~~1:1000 FC~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | MYO1A Antibody (Center S291) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | MYO1A |
---|---|
Synonyms | MYHL |
Function | Involved in directing the movement of organelles along actin filaments. |
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Provided below are standard protocols that you may find useful for product applications.
Background
MYO1A belongs to the myosin superfamily. Myosins are molecular motors that, upon interaction with actin filaments, utilize energy from ATP hydrolysis to generate mechanical force. Each myosin has a conserved N-terminal motor domain that contains both ATP-binding and actin-binding sequences. Following the motor domain is a light-chain-binding 'neck' region containing 1-6 copies of a repeat element, the IQ motif, that serves as a binding site for calmodulin or other members of the EF-hand superfamily of calcium-binding proteins. At the C-terminus, each myosin class has a distinct tail domain that serves in dimerization, membrane binding, protein binding, and/or enzymatic activities and targets each myosin to its particular subcellular location. The kidney epithelial cell line, LLC-PK1-CL4 (CL4), forms a well ordered brush border (BB) on its apical surface. Experiments indicate that the brush border population of the protein turns over rapidly, while its head and tail domains interact transiently with the core actin and plasma membrane, respectively. A rapidly exchanging pool of the protein envelops an actin core bundle that, by comparison, is static in structure.
References
D'Adamo,P., Pinna,M. Hum. Genet. 112 (3), 319-320 (2003)
Hoshimaru,M., Fujio,Y. J. Biochem. 106 (3), 455-459 (1989)
Durrbach,A., Collins,K. Proc. Natl. Acad. Sci. U.S.A. 93 (14), 7053-7058 (1996)
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