|Application ||WB, FC, E|
|Calculated MW||49097 Da|
|Antigen Region||209-236 aa|
|Other Names||Histone-lysine N-methyltransferase SMYD3, SET and MYND domain-containing protein 3, Zinc finger MYND domain-containing protein 1, SMYD3, ZMYND1, ZNFN3A1|
|Target/Specificity||This SMYD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 209-236 amino acids from the Central region of human SMYD3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SMYD3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Histone methyltransferase. Specifically methylates 'Lys- 4' of histone H3, inducing di- and tri-methylation, but not monomethylation (PubMed:15235609, PubMed:22419068). Also methylates 'Lys-5' of histone H4 (PubMed:22419068). Plays an important role in transcriptional activation as a member of an RNA polymerase complex (PubMed:15235609). Binds DNA containing 5'- CCCTCC-3' or 5'-GAGGGG-3' sequences (PubMed:15235609).|
|Cellular Location||Cytoplasm. Nucleus. Note=Mainly cytoplasmic when cells are arrested at G0/G1. Accumulates in the nucleus at S phase and G2/M.|
|Tissue Location||Expressed in skeletal muscles and testis. Overexpressed in a majority of colorectal and hepatocellular carcinomas.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
SMYD3 is a histone methyltransferase that plays a role in transcriptional regulation as a member of an RNA polymerase complex.
Zou,J.N., Wang,S.Z. Cancer Lett. 280 (1), 78-85 (2009)
Wang,S.Z., Luo,X.G. BMB Rep 41 (4), 294-299 (2008)
Wang,H., Liu,Y. Cancer Sci. 99 (4), 787-791 (2008)
Barlesi,F., Giaccone,G. Int. J. Cancer 122 (6), 1441-1442 (2008)
Hamamoto,R., Silva,F.P. Cancer Sci. 97 (2), 113-118 (2006)
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