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TYR Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - TYR  Antibody (Center) AP7345c
    Confocal immunofluorescent analysis of TYR Antibody (Center)(Cat#AP7345c) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
    detail
  • WB - TYR  Antibody (Center) AP7345c
    Western blot analysis of Tyrosinase Antibody (Center) (Cat.#AP7345c) in A375, 293 cell line lysates (35ug/lane). Tyrosinase (arrow) was detected using the purified Pab.
    detail
  • IHC-P - TYR  Antibody (Center) AP7345c
    TYR Antibody (Center) (AP7345c)immunohistochemistry analysis in formalin fixed and paraffin embedded human melanoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TYR Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, IF, WB, E
Primary Accession P14679
Other Accession P11344
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 60393 Da
Antigen Region 176-205 aa
Additional Information
Gene ID 7299
Other Names Tyrosinase, LB24-AB, Monophenol monooxygenase, SK29-AB, Tumor rejection antigen AB, TYR
Target/Specificity This TYR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 176-205 amino acids from the Central region of human TYR.
Dilution IF~~1:10~50
WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTYR Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TYR (HGNC:12442)
Function This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine (By similarity). In addition to hydroxylating tyrosine to DOPA (3,4- dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA- quinone, and possibly the oxidation of DHI (5,6-dihydroxyindole) to indole-5,6 quinone (PubMed:28661582).
Cellular Location Melanosome membrane; Single-pass type I membrane protein. Melanosome {ECO:0000250|UniProtKB:P11344}. Note=Proper trafficking to melanosome is regulated by SGSM2, ANKRD27, RAB9A, RAB32 and RAB38 {ECO:0000250|UniProtKB:P11344}
Research Areas
Citations (0)
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Background

TYR catalyzes the first 2 steps, and at least 1 subsequent step, in the conversion of tyrosine to melanin. The protein has both tyrosine hydroxylase and dopa oxidase catalytic activities, and requires copper for function. Mutations in this protein result in oculocutaneous albinism, and nonpathologic polymorphisms result in skin pigmentation variation.

References

Ostankovitch,M. J. Immunol. 182 (8), 4830-4835 (2009)
Chintamaneni,C.D. Proc. Natl. Acad. Sci. U.S.A. 88 (12), 5272-5276 (1991)

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$ 182.50
$ 70.00
Cat# AP7345c
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