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BF Antibody (Center)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP7357c | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
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BF Antibody (Center) - Product info | |
| Application | WB, IHC
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| Primary Accession | P00751 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 85533 Da |
BF Antibody (Center) - Additional info | |
| Gene ID 629 | |
| Other Names CFB; BF; BFD; Complement factor B; C3/C5 convertase; Glycine-rich beta glycoprotein; PBF2; Properdin factor B; Complement factor B Ba fragment; Complement factor B Bb fragment | |
| Target/Specificity This BF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 401~431 amino acids from the Center region of human BF. | |
| Dilution WB~~1:50~100WB~~1:1000 IHC~~1:50~100 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions BF Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. | |
BF Antibody (Center) - Protein Information | |
| Name CFB | |
| Synonyms BF, BFD | |
| Function Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B- lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes Ba inhibits the proliferation of preactivated B-lymphocytes | |
| Cellular Location Secreted. | |
BF Antibody (Center) - Related products
AP9036c: CFB Antibody (Center)
BF Antibody (Center) - Application data
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Western blot analysis of BF Antibody (Center) (Cat.# AP7357c) in HepG2 cell line and mouse liver tissue lysates (35ug/lane). BF (arrow) was detected using the purified Pab.
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BF Antibody (Center) (RB18685) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BF Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
BF Antibody (Center) - Research Areas
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Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
BF is complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation.
REFERENCES
Baatz,H., Klin Monatsbl Augenheilkd 226 (8), 654-658 (2009) Goring,K., Am. J. Physiol., Cell Physiol. 296 (5), C1140-C1150 (2009)
