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COL5A2 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - COL5A2 Antibody (N-term) AP7358a
    Western blot analysis of COL5A2 Antibody (N-term) (Cat.# AP7358a) in mouse lung tissue lysates (35ug/lane). COL5A2 (arrow) was detected using the purified Pab.
    detail
  • FC - COL5A2 Antibody (N-term) AP7358a
    COL5A2 Antibody (N-term) (Cat. #AP7358a) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, WB, E
Primary Accession P05997
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 144910 Da
Antigen Region 90-118 aa
Additional Information
Gene ID 1290
Other Names Collagen alpha-2(V) chain, COL5A2
Target/Specificity This COL5A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 90-118 amino acids from the N-terminal region of human COL5A2.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCOL5A2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name COL5A2
Function Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. Type V collagen is a key determinant in the assembly of tissue-specific matrices (By similarity).
Cellular Location Secreted, extracellular space, extracellular matrix {ECO:0000255|PROSITE-ProRule:PRU00793}
Research Areas
Citations (0)
citation

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Background

COL5A2 is an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This protein is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations.

References

Menon,R., Reprod. Biol. Endocrinol. 7 (1), 62 (2009)
Videman,T., Arthritis Rheum. 60 (2), 470-481 (2009)
Segev,F., Invest. Ophthalmol. Vis. Sci. 47 (2), 565-573 (2006)

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$ 365.00
$ 140.00
Cat# AP7358a
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