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RAD50 Antibody (N-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP7360a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
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- Backgrounds
RAD50 Antibody (N-term) - Product info | |
| Application | WB, FC
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| Primary Accession | Q92878 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 153892 Da |
RAD50 Antibody (N-term) - Additional info | |
| Gene ID 10111 | |
| Other Names RAD50; DNA repair protein RAD50 | |
| Target/Specificity This RAD50 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100~129 amino acids from the N-terminal region of human RAD50. | |
| Dilution WB~~1:50~100WB~~1:1000 FC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions RAD50 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
RAD50 Antibody (N-term) - Protein Information | |
| Name RAD50 | |
| Function Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand- specific 3'-5' exonuclease activity, which are provided by MRE11A RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation | |
| Cellular Location Nucleus. Chromosome, telomere. Note=Localizes to discrete nuclear foci after treatment with genotoxic agents | |
| Tissue Location Expressed at very low level in most tissues, except in testis where it is expressed at higher level. Expressed in fibroblasts | |
RAD50 Antibody (N-term) - Related products
AP7360a: RAD50 Antibody (N-term)
RAD50 Antibody (N-term) - Application data
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Western blot analysis of RAD50 Antibody (N-term) (Cat.# AP7360a) in K562 cell line lysates (35ug/lane). RAD50 (arrow) was detected using the purified Pab.
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RAD50 Antibody (N-term) (Cat. #AP7360a) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
RAD50 Antibody (N-term) - Research Areas
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BACKGROUND
The protein is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest RAD50 gene is essential for cell growth and viability.
REFERENCES
Pugh,T.J., Clin. Cancer Res. 15 (15), 5008-5016 (2009) Karen,K.A., J. Virol. 83 (9), 4565-4573 (2009)
