MTHFD2 Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| FC, IHC-P, WB, E |
---|---|
Primary Accession | P13995 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 37895 Da |
Antigen Region | 271-299 aa |
Gene ID | 10797 |
---|---|
Other Names | Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial, NAD-dependent methylenetetrahydrofolate dehydrogenase, Methenyltetrahydrofolate cyclohydrolase, MTHFD2, NMDMC |
Target/Specificity | This MTHFD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 271-299 amino acids from the C-terminal region of human MTHFD2. |
Dilution | WB~~1:1000 IHC-P~~1:10~50 FC~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | MTHFD2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | MTHFD2 |
---|---|
Synonyms | NMDMC |
Function | Although its dehydrogenase activity is NAD-specific, it can also utilize NADP at a reduced efficiency. |
Cellular Location | Mitochondrion. |
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Provided below are standard protocols that you may find useful for product applications.
Background
MTHFD2 is a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD.
References
Wang,S.S., Leukemia 23 (3), 596-602 (2009)
Pawelek,P.D., Biochemistry 37 (4), 1109-1115 (1998)
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