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COL4A1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - COL4A1 Antibody (N-term) AP7369a
    All lanes : Anti-COL4A1 Antibody (N-term) at 1:1000 dilution Lane 1: A549 whole cell lysate Lane 2: DU145 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: HT-1080 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 161 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IF - COL4A1 Antibody (N-term) AP7369a
    Fluorescent image of U251 cells stained with COL4A1 Antibody (N-term) (Cat#AP7369A). AP7369A was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
  • WB - COL4A1 Antibody (N-term) AP7369a
    Western blot analysis of lysates from Hela cell line and human placenta tissue lysate(from left to right), using COL4A1 Antibody (N-term)(Cat. #AP7369A). AP7369A was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P02462
Other Accession P02463
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 160615 Da
Antigen Region 27-54 aa
Additional Information
Gene ID 1282
Other Names Collagen alpha-1(IV) chain, Arresten, COL4A1
Target/Specificity This COL4A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-54 amino acids from the N-terminal region of human COL4A1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCOL4A1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name COL4A1
Function Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
Cellular Location Secreted, extracellular space, extracellular matrix, basement membrane
Tissue Location Highly expressed in placenta.
Research Areas
Citations ( 0 )


COL4A1 is the major type IV alpha collagen chain of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter.


de Vries,L.S., Ann. Neurol. 65 (1), 12-18 (2009)
Mazouni,C., Br. J. Cancer 99 (1), 68-71 (2008)

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$ 295.00
$ 99.00
Cat# AP7369a
(40 western blots)
Availability: In Stock
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